Restriction map for
pFN21AE1692
(Linear) (Six-base) MAPSORT of: KIEE1692.seq Check: 9475 from: 1 to: 600
KIEE1692.orf
With 182 enzymes: SgfI *
March 22, 2012 15:53 ..
AatII G_ACGT'C
Cuts at: 0 338 600
Size: 338 262
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 600
Size: 316 284
AlwNI CAG_nnn'CTG
Cuts at: 0 61 600
Size: 61 539
ApaI G_GGCC'C
Cuts at: 0 419 600
Size: 419 181
AvaI C'yCGr_G
Cuts at: 0 220 425 600
Size: 220 205 175
Fragments arranged by size:
220 205 175
BanII G_rGCy'C
Cuts at: 0 419 600
Size: 419 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 207 600
Size: 207 393
BbvCI CC'TCA_GC
Cuts at: 0 401 600
Size: 401 199
BciVI GTATCCnnnnn_n'
Cuts at: 0 403 600
Size: 403 197
BglI GCCn_nnn'nGGC
Cuts at: 0 107 274 600
Size: 107 167 326
Fragments arranged by size:
326 167 107
BlpI GC'TnA_GC
Cuts at: 0 278 600
Size: 278 322
Bme1580I G_kGCm'C
Cuts at: 0 419 600
Size: 419 181
BmrI ACTGGGnnnn_n'
Cuts at: 0 98 600
Size: 98 502
BmtI G_CTAG'C
Cuts at: 0 142 600
Size: 142 458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 229 325 349 367 442 514 600
Size: 216 13 96 24 18 75 72 86
Fragments arranged by size:
216 96 86 75 72 24 18 13
Bpu10I CC'TnA_GC
Cuts at: 0 401 600
Size: 401 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 600
Size: 166 434
BsaI GGTCTCn'nnnn_
Cuts at: 0 530 600
Size: 530 70
BsaHI Gr'CG_yC
Cuts at: 0 164 335 600
Size: 164 171 265
Fragments arranged by size:
265 171 164
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 600
Size: 358 30 212
Fragments arranged by size:
358 212 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 517 600
Size: 517 83
BseYI C'CCAG_C
Cuts at: 0 321 377 600
Size: 321 56 223
Fragments arranged by size:
321 223 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 433 600
Size: 346 87 167
Fragments arranged by size:
346 167 87
Bsp1286I G_dGCh'C
Cuts at: 0 419 600
Size: 419 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 101 600
Size: 101 499
BsrFI r'CCGG_y
Cuts at: 0 452 600
Size: 452 148
Bsu36I CC'TnA_GG
Cuts at: 0 235 600
Size: 235 365
BtgI C'CryG_G
Cuts at: 0 17 50 600
Size: 17 33 550
Fragments arranged by size:
550 33 17
EaeI y'GGCC_r
Cuts at: 0 47 551 600
Size: 47 504 49
Fragments arranged by size:
504 49 47
EarI CTCTTCn'nnn_
Cuts at: 0 167 523 600
Size: 167 356 77
Fragments arranged by size:
356 167 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 511 600
Size: 511 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 229 316 325 349 367 442 514
Size: 216 13 87 9 24 18 75 72
Cuts at: 514 600
Size: 86
Fragments arranged by size:
216 87 86 75 72 24 18 13
9
EcoO109I rG'GnC_Cy
Cuts at: 0 58 231 300 415 422 465 600
Size: 58 173 69 115 7 43 135
Fragments arranged by size:
173 135 115 69 58 43 7
HaeII r_GCGC'y
Cuts at: 0 261 600
Size: 261 339
HincII GTy'rAC
Cuts at: 0 581 600
Size: 581 19
MspA1I CmG'CkG
Cuts at: 0 313 484 600
Size: 313 171 116
Fragments arranged by size:
313 171 116
NheI G'CTAG_C
Cuts at: 0 138 600
Size: 138 462
PfoI T'CCnGG_A
Cuts at: 0 302 458 600
Size: 302 156 142
Fragments arranged by size:
302 156 142
PpuMI rG'GwC_Cy
Cuts at: 0 231 300 600
Size: 231 69 300
Fragments arranged by size:
300 231 69
PspOMI G'GGCC_C
Cuts at: 0 415 600
Size: 415 185
PstI C_TGCA'G
Cuts at: 0 9 600
Size: 9 591
SfcI C'TryA_G
Cuts at: 0 5 600
Size: 5 595
SmaI CCC'GGG
Cuts at: 0 222 427 600
Size: 222 205 173
Fragments arranged by size:
222 205 173
SmlI C'TyrA_G
Cuts at: 0 181 600
Size: 181 419
StuI AGG'CCT
Cuts at: 0 388 600
Size: 388 212
StyI C'CwwG_G
Cuts at: 0 167 269 600
Size: 167 102 331
Fragments arranged by size:
331 167 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 358 390 577 600
Size: 358 32 187 23
Fragments arranged by size:
358 187 32 23
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 600
Size: 165 435
XmaI C'CCGG_G
Cuts at: 0 220 425 600
Size: 220 205 175
Fragments arranged by size:
220 205 175
ZraI GAC'GTC
Cuts at: 0 336 600
Size: 336 264
Enzymes that do cut:
AatII AcuI AlwNI ApaI AvaI BanII BbsI BbvCI
BciVI BglI BlpI Bme1580I BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI Bsp1286I
BspMI BsrFI Bsu36I BtgI EaeI EarI EciI Eco57MI
EcoO109I HaeII HincII MspA1I NheI PfoI PpuMI PspOMI
PstI SfcI SmaI SmlI StuI StyI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BclI
BfrBI BglII BmgBI BplI BsaAI BsaBI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI
XhoI XmnI