Restriction map for
pFN21AE1691
(Linear) (Six-base) MAPSORT of: KIEE1691.seq Check: 3650 from: 1 to: 600
KIEE1691.orf
With 182 enzymes: SgfI *
March 22, 2012 15:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 209 258 600
Size: 209 49 342
Fragments arranged by size:
342 209 49
AatII G_ACGT'C
Cuts at: 0 365 600
Size: 365 235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 337 600
Size: 229 108 263
Fragments arranged by size:
263 229 108
AleI CACnn'nnGTG
Cuts at: 0 31 600
Size: 31 569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 49 135 600
Size: 49 86 465
Fragments arranged by size:
465 86 49
AlwNI CAG_nnn'CTG
Cuts at: 0 79 159 248 600
Size: 79 80 89 352
Fragments arranged by size:
352 89 80 79
ApaI G_GGCC'C
Cuts at: 0 446 600
Size: 446 154
AvaI C'yCGr_G
Cuts at: 0 452 520 600
Size: 452 68 80
Fragments arranged by size:
452 80 68
BanI G'GyrC_C
Cuts at: 0 221 600
Size: 221 379
BanII G_rGCy'C
Cuts at: 0 102 446 600
Size: 102 344 154
Fragments arranged by size:
344 154 102
BbvCI CC'TCA_GC
Cuts at: 0 428 600
Size: 428 172
BciVI GTATCCnnnnn_n'
Cuts at: 0 430 600
Size: 430 170
BlpI GC'TnA_GC
Cuts at: 0 299 600
Size: 299 301
Bme1580I G_kGCm'C
Cuts at: 0 446 600
Size: 446 154
BmrI ACTGGGnnnn_n'
Cuts at: 0 26 126 399 600
Size: 26 100 273 201
Fragments arranged by size:
273 201 100 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 346 469 600
Size: 89 257 123 131
Fragments arranged by size:
257 131 123 89
Bpu10I CC'TnA_GC
Cuts at: 0 428 600
Size: 428 172
BsaI GGTCTCn'nnnn_
Cuts at: 0 557 600
Size: 557 43
BsaHI Gr'CG_yC
Cuts at: 0 362 600
Size: 362 238
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 544 600
Size: 544 56
BseYI C'CCAG_C
Cuts at: 0 348 600
Size: 348 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 373 600
Size: 9 364 227
Fragments arranged by size:
364 227 9
Bsp1286I G_dGCh'C
Cuts at: 0 102 446 600
Size: 102 344 154
Fragments arranged by size:
344 154 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 209 258 600
Size: 209 49 342
Fragments arranged by size:
342 209 49
BsrFI r'CCGG_y
Cuts at: 0 479 600
Size: 479 121
BstXI CCAn_nnnn'nTGG
Cuts at: 0 291 600
Size: 291 309
Bsu36I CC'TnA_GG
Cuts at: 0 256 600
Size: 256 344
BtgI C'CryG_G
Cuts at: 0 93 284 434 600
Size: 93 191 150 166
Fragments arranged by size:
191 166 150 93
BtsI GCAGTG_nn'
Cuts at: 0 59 600
Size: 59 541
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 600
Size: 595 5
EaeI y'GGCC_r
Cuts at: 0 47 600
Size: 47 553
EarI CTCTTCn'nnn_
Cuts at: 0 188 241 550 600
Size: 188 53 309 50
Fragments arranged by size:
309 188 53 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 538 600
Size: 538 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 229 337 346 469 600
Size: 89 140 108 9 123 131
Fragments arranged by size:
140 131 123 108 89 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 171 600
Size: 171 429
EcoO109I rG'GnC_Cy
Cuts at: 0 245 252 442 449 492 588 600
Size: 245 7 190 7 43 96 12
Fragments arranged by size:
245 190 96 43 12 7 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 217 600
Size: 217 383
HaeII r_GCGC'y
Cuts at: 0 282 600
Size: 282 318
MslI CAynn'nnrTG
Cuts at: 0 31 600
Size: 31 569
MspA1I CmG'CkG
Cuts at: 0 264 337 600
Size: 264 73 263
Fragments arranged by size:
264 263 73
NcoI C'CATG_G
Cuts at: 0 284 600
Size: 284 316
PflMI CCAn_nnn'nTGG
Cuts at: 0 248 600
Size: 248 352
PfoI T'CCnGG_A
Cuts at: 0 104 485 600
Size: 104 381 115
Fragments arranged by size:
381 115 104
PpuMI rG'GwC_Cy
Cuts at: 0 245 252 449 588 600
Size: 245 7 197 139 12
Fragments arranged by size:
245 197 139 12 7
PspOMI G'GGCC_C
Cuts at: 0 442 600
Size: 442 158
PspXI vC'TCGA_Gb
Cuts at: 0 520 600
Size: 520 80
PstI C_TGCA'G
Cuts at: 0 164 220 269 600
Size: 164 56 49 331
Fragments arranged by size:
331 164 56 49
PvuII CAG'CTG
Cuts at: 0 264 600
Size: 264 336
SfcI C'TryA_G
Cuts at: 0 160 216 265 600
Size: 160 56 49 335
Fragments arranged by size:
335 160 56 49
SmaI CCC'GGG
Cuts at: 0 454 600
Size: 454 146
SmlI C'TyrA_G
Cuts at: 0 520 600
Size: 520 80
StuI AGG'CCT
Cuts at: 0 166 526 600
Size: 166 360 74
Fragments arranged by size:
360 166 74
StyI C'CwwG_G
Cuts at: 0 284 600
Size: 284 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 205 237 600
Size: 205 32 363
Fragments arranged by size:
363 205 32
XhoI C'TCGA_G
Cuts at: 0 520 600
Size: 520 80
XmaI C'CCGG_G
Cuts at: 0 452 600
Size: 452 148
ZraI GAC'GTC
Cuts at: 0 363 600
Size: 363 237
Enzymes that do cut:
AarI AatII AcuI AleI AlfI AlwNI ApaI AvaI
BanI BanII BbvCI BciVI BlpI Bme1580I BmrI BpmI
Bpu10I BsaI BsaHI BseRI BseYI BsgI Bsp1286I BspMI
BsrFI BstXI Bsu36I BtgI BtsI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI HaeII MslI MspA1I
NcoI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII
SfcI SmaI SmlI StuI StyI TstI XhoI XmaI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BclI BfrBI BglI BglII
BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EcoICRI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I
XbaI XcmI XmnI