Restriction map for
pFN21AE1685
(Linear) (Six-base) MAPSORT of: KIEE1685.seq Check: 2617 from: 1 to: 591
KIEE1685.orf
With 182 enzymes: SgfI *
July 3, 2012 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 158 577 591
Size: 158 419 14
Fragments arranged by size:
419 158 14
Acc65I G'GTAC_C
Cuts at: 0 94 591
Size: 94 497
AfeI AGC'GCT
Cuts at: 0 310 591
Size: 310 281
AleI CACnn'nnGTG
Cuts at: 0 571 591
Size: 571 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 589 591
Size: 589 2
AlwNI CAG_nnn'CTG
Cuts at: 0 369 398 462 591
Size: 369 29 64 129
Fragments arranged by size:
369 129 64 29
AvaI C'yCGr_G
Cuts at: 0 158 501 591
Size: 158 343 90
Fragments arranged by size:
343 158 90
BanI G'GyrC_C
Cuts at: 0 77 94 163 591
Size: 77 17 69 428
Fragments arranged by size:
428 77 69 17
BanII G_rGCy'C
Cuts at: 0 509 591
Size: 509 82
BbvCI CC'TCA_GC
Cuts at: 0 116 246 591
Size: 116 130 345
Fragments arranged by size:
345 130 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 412 446 591
Size: 412 34 145
Fragments arranged by size:
412 145 34
Bme1580I G_kGCm'C
Cuts at: 0 80 265 591
Size: 80 185 326
Fragments arranged by size:
326 185 80
BmgBI CAC'GTC
Cuts at: 0 552 591
Size: 552 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 252 591
Size: 252 339
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 261 591
Size: 261 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 345 439 591
Size: 229 116 94 152
Fragments arranged by size:
229 152 116 94
Bpu10I CC'TnA_GC
Cuts at: 0 116 246 591
Size: 116 130 345
Fragments arranged by size:
345 130 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 271 295 301 318 325 348 591
Size: 271 24 6 17 7 23 243
Fragments arranged by size:
271 243 24 23 17 7 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 591
Size: 295 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 411 549 591
Size: 22 389 138 42
Fragments arranged by size:
389 138 42 22
BsiEI CG_ry'CG
Cuts at: 0 277 591
Size: 277 314
Bsp1286I G_dGCh'C
Cuts at: 0 80 265 509 591
Size: 80 185 244 82
Fragments arranged by size:
244 185 82 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 577 591
Size: 158 419 14
Fragments arranged by size:
419 158 14
BsrFI r'CCGG_y
Cuts at: 0 479 591
Size: 479 112
BssSI C'ACGA_G
Cuts at: 0 235 591
Size: 235 356
BstAPI GCAn_nnn'nTGC
Cuts at: 0 183 591
Size: 183 408
Bsu36I CC'TnA_GG
Cuts at: 0 70 591
Size: 70 521
BtsI GCAGTG_nn'
Cuts at: 0 102 591
Size: 102 489
ClaI AT'CG_AT
Cuts at: 0 404 591
Size: 404 187
EaeI y'GGCC_r
Cuts at: 0 132 274 591
Size: 132 142 317
Fragments arranged by size:
317 142 132
EagI C'GGCC_G
Cuts at: 0 274 591
Size: 274 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 345 439 591
Size: 229 116 94 152
Fragments arranged by size:
229 152 116 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 69 133 591
Size: 69 64 458
Fragments arranged by size:
458 69 64
EcoO109I rG'GnC_Cy
Cuts at: 0 138 242 521 591
Size: 138 104 279 70
Fragments arranged by size:
279 138 104 70
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 390 591
Size: 390 201
HaeII r_GCGC'y
Cuts at: 0 312 591
Size: 312 279
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 386 418 591
Size: 386 32 173
Fragments arranged by size:
386 173 32
KpnI G_GTAC'C
Cuts at: 0 98 591
Size: 98 493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 591
Size: 269 322
MslI CAynn'nnrTG
Cuts at: 0 571 591
Size: 571 20
MspA1I CmG'CkG
Cuts at: 0 430 591
Size: 430 161
NaeI GCC'GGC
Cuts at: 0 481 591
Size: 481 110
NgoMIV G'CCGG_C
Cuts at: 0 479 591
Size: 479 112
NspI r_CATG'y
Cuts at: 0 47 591
Size: 47 544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 113 145 591
Size: 113 32 446
Fragments arranged by size:
446 113 32
PspXI vC'TCGA_Gb
Cuts at: 0 158 591
Size: 158 433
PvuII CAG'CTG
Cuts at: 0 430 591
Size: 430 161
SfcI C'TryA_G
Cuts at: 0 188 591
Size: 188 403
SmlI C'TyrA_G
Cuts at: 0 158 591
Size: 158 433
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 591
Size: 316 275
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 295 318 327 350 591
Size: 295 23 9 23 241
Fragments arranged by size:
295 241 23 23 9
XhoI C'TCGA_G
Cuts at: 0 158 591
Size: 158 433
Enzymes that do cut:
AarI Acc65I AfeI AleI AlfI AlwNI AvaI BanI
BanII BbvCI BcgI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaXI BseRI BsgI BsiEI Bsp1286I BspMI BsrFI
BssSI BstAPI Bsu36I BtsI ClaI EaeI EagI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KpnI MmeI MslI
MspA1I NaeI NgoMIV NspI PpiI PspXI PvuII SfcI
SmlI TaqII TstI XhoI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI BmtI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI CspCI DraI DraIII DrdI EarI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI Tth111I XbaI XcmI XmaI XmnI ZraI