Restriction map for
pFN21AE1683
(Linear) (Six-base) MAPSORT of: KIEE1683.seq Check: 9171 from: 1 to: 588
KIEE1683.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 354 357 588
Size: 354 3 231
Fragments arranged by size:
354 231 3
AvaI C'yCGr_G
Cuts at: 0 93 588
Size: 93 495
BanI G'GyrC_C
Cuts at: 0 108 438 588
Size: 108 330 150
Fragments arranged by size:
330 150 108
BanII G_rGCy'C
Cuts at: 0 102 247 588
Size: 102 145 341
Fragments arranged by size:
341 145 102
BbeI G_GCGC'C
Cuts at: 0 112 588
Size: 112 476
BbvCI CC'TCA_GC
Cuts at: 0 122 588
Size: 122 466
Bpu10I CC'TnA_GC
Cuts at: 0 76 122 588
Size: 76 46 466
Fragments arranged by size:
466 76 46
BsaHI Gr'CG_yC
Cuts at: 0 109 588
Size: 109 479
BsaWI w'CCGG_w
Cuts at: 0 441 588
Size: 441 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 261 268 478 490 588
Size: 223 38 7 210 12 98
Fragments arranged by size:
223 210 98 38 12 7
BseYI C'CCAG_C
Cuts at: 0 167 588
Size: 167 421
BsiEI CG_ry'CG
Cuts at: 0 187 251 323 588
Size: 187 64 72 265
Fragments arranged by size:
265 187 72 64
BsiHKAI G_wGCw'C
Cuts at: 0 102 588
Size: 102 486
BsmI GAATG_Cn'
Cuts at: 0 420 588
Size: 420 168
Bsp1286I G_dGCh'C
Cuts at: 0 102 247 588
Size: 102 145 341
Fragments arranged by size:
341 145 102
BsrBI CCG'CTC
Cuts at: 0 253 588
Size: 253 335
BsrFI r'CCGG_y
Cuts at: 0 87 186 283 588
Size: 87 99 97 305
Fragments arranged by size:
305 99 97 87
BstAPI GCAn_nnn'nTGC
Cuts at: 0 354 357 588
Size: 354 3 231
Fragments arranged by size:
354 231 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 480 588
Size: 480 108
BstYI r'GATC_y
Cuts at: 0 483 588
Size: 483 105
BtgI C'CryG_G
Cuts at: 0 23 81 386 588
Size: 23 58 305 202
Fragments arranged by size:
305 202 58 23
EaeI y'GGCC_r
Cuts at: 0 184 248 426 521 588
Size: 184 64 178 95 67
Fragments arranged by size:
184 178 95 67 64
EagI C'GGCC_G
Cuts at: 0 184 248 588
Size: 184 64 340
Fragments arranged by size:
340 184 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 211 588
Size: 211 377
EcoICRI GAG'CTC
Cuts at: 0 100 588
Size: 100 488
HaeII r_GCGC'y
Cuts at: 0 73 112 372 410 588
Size: 73 39 260 38 178
Fragments arranged by size:
260 178 73 39 38
KasI G'GCGC_C
Cuts at: 0 108 588
Size: 108 480
MscI TGG'CCA
Cuts at: 0 523 588
Size: 523 65
MspA1I CmG'CkG
Cuts at: 0 12 25 83 293 354 388 588
Size: 12 13 58 210 61 34 200
Fragments arranged by size:
210 200 61 58 34 13 12
NaeI GCC'GGC
Cuts at: 0 285 588
Size: 285 303
NarI GG'CG_CC
Cuts at: 0 109 588
Size: 109 479
NgoMIV G'CCGG_C
Cuts at: 0 283 588
Size: 283 305
PfoI T'CCnGG_A
Cuts at: 0 503 588
Size: 503 85
PstI C_TGCA'G
Cuts at: 0 350 588
Size: 350 238
PvuII CAG'CTG
Cuts at: 0 354 588
Size: 354 234
RsrII CG'GwC_CG
Cuts at: 0 458 588
Size: 458 130
SacI G_AGCT'C
Cuts at: 0 102 588
Size: 102 486
SacII CC_GC'GG
Cuts at: 0 26 84 389 588
Size: 26 58 305 199
Fragments arranged by size:
305 199 58 26
ScaI AGT'ACT
Cuts at: 0 214 588
Size: 214 374
SfcI C'TryA_G
Cuts at: 0 346 588
Size: 346 242
SfoI GGC'GCC
Cuts at: 0 110 588
Size: 110 478
SgrAI Cr'CCGG_yG
Cuts at: 0 283 588
Size: 283 305
SmaI CCC'GGG
Cuts at: 0 95 588
Size: 95 493
StyI C'CwwG_G
Cuts at: 0 128 588
Size: 128 460
TatI w'GTAC_w
Cuts at: 0 212 588
Size: 212 376
XmaI C'CCGG_G
Cuts at: 0 93 588
Size: 93 495
Enzymes that do cut:
AlwNI AvaI BanI BanII BbeI BbvCI Bpu10I BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BsrBI
BsrFI BstAPI BstXI BstYI BtgI EaeI EagI EciI
EcoICRI HaeII KasI MscI MspA1I NaeI NarI NgoMIV
PfoI PstI PvuII RsrII SacI SacII ScaI SfcI
SfoI SgrAI SmaI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaXI BsgI BsiWI BsmBI BspEI BspHI BspMI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MslI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI SalI
SanDI SapI SbfI SexAI SfiI SgfI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmnI ZraI