(Linear) (Six-base) MAPSORT of: KIEE1680.seq  Check: 8508  from: 1  to: 582



KIEE1680.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

Acc65I G'GTAC_C

Cuts at:      0     224     582 
   Size:        224     358
 
ApoI r'AATT_y

Cuts at:      0     346     582 
   Size:        346     236
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     208     241     582 
   Size:        208      33     341

  Fragments arranged by size: 

                 341     208      33
 
BamHI G'GATC_C

Cuts at:      0      23     582 
   Size:         23     559
 
BanI G'GyrC_C

Cuts at:      0     224     582 
   Size:        224     358
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     207     582 
   Size:        207     375
 
BciVI GTATCCnnnnn_n'

Cuts at:      0       5     582 
   Size:          5     577
 
BfrBI ATG'CAT

Cuts at:      0     454     582 
   Size:        454     128
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     582 
   Size:         92     490
 
Bpu10I CC'TnA_GC

Cuts at:      0     146     582 
   Size:        146     436
 
BsmI GAATG_Cn'

Cuts at:      0     400     582 
   Size:        400     182
 
BsrDI GCAATG_nn'

Cuts at:      0     102     365     405     582 
   Size:        102     263      40     177

  Fragments arranged by size: 

                 263     177     102      40
 
BstYI r'GATC_y

Cuts at:      0      23     582 
   Size:         23     559
 
Bsu36I CC'TnA_GG

Cuts at:      0     229     582 
   Size:        229     353
 
ClaI AT'CG_AT

Cuts at:      0     236     582 
   Size:        236     346
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     209     582 
   Size:        209     373
 
EarI CTCTTCn'nnn_

Cuts at:      0     211     582 
   Size:        211     371
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     582 
   Size:         92     490
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     521     582 
   Size:        521      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0     260     582 
   Size:        260     322
 
EcoRI G'AATT_C

Cuts at:      0     346     582 
   Size:        346     236
 
KpnI G_GTAC'C

Cuts at:      0     228     582 
   Size:        228     354
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     582 
   Size:        294     288
 
NdeI CA'TA_TG

Cuts at:      0     450     582 
   Size:        450     132
 
NsiI A_TGCA'T

Cuts at:      0     456     582 
   Size:        456     126
 
PpuMI rG'GwC_Cy

Cuts at:      0     260     582 
   Size:        260     322
 
SanDI GG'GwC_CC

Cuts at:      0     260     582 
   Size:        260     322
 
SfcI C'TryA_G

Cuts at:      0      42     582 
   Size:         42     540
 
XmnI GAAnn'nnTTC

Cuts at:      0     425     582 
   Size:        425     157
 

 Enzymes that do cut:

   Acc65I     ApoI     BaeI    BamHI     BanI     BbsI    BciVI    BfrBI
     BpmI   Bpu10I     BsmI    BsrDI    BstYI   Bsu36I     ClaI     DrdI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     KpnI     MmeI     NdeI
     NsiI    PpuMI    SanDI     SfcI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BanII     BbeI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI