Restriction map for
pFN21AE1679
(Linear) (Six-base) MAPSORT of: KIEE1679.seq Check: 6426 from: 1 to: 579
KIEE1679.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 216 312 512 579
Size: 216 96 200 67
Fragments arranged by size:
216 200 96 67
AvaI C'yCGr_G
Cuts at: 0 447 579
Size: 447 132
BanI G'GyrC_C
Cuts at: 0 241 579
Size: 241 338
BanII G_rGCy'C
Cuts at: 0 473 579
Size: 473 106
BbeI G_GCGC'C
Cuts at: 0 245 579
Size: 245 334
BbvCI CC'TCA_GC
Cuts at: 0 358 579
Size: 358 221
BglI GCCn_nnn'nGGC
Cuts at: 0 393 458 579
Size: 393 65 121
Fragments arranged by size:
393 121 65
BlpI GC'TnA_GC
Cuts at: 0 256 279 579
Size: 256 23 300
Fragments arranged by size:
300 256 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 492 579
Size: 492 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 543 579
Size: 543 36
Bpu10I CC'TnA_GC
Cuts at: 0 358 579
Size: 358 221
BsaI GGTCTCn'nnnn_
Cuts at: 0 66 124 579
Size: 66 58 455
Fragments arranged by size:
455 66 58
BsaAI yAC'GTr
Cuts at: 0 228 579
Size: 228 351
BsaHI Gr'CG_yC
Cuts at: 0 242 329 579
Size: 242 87 250
Fragments arranged by size:
250 242 87
BsaWI w'CCGG_w
Cuts at: 0 133 579
Size: 133 446
BsiEI CG_ry'CG
Cuts at: 0 118 152 379 416 579
Size: 118 34 227 37 163
Fragments arranged by size:
227 163 118 37 34
BsiHKAI G_wGCw'C
Cuts at: 0 326 579
Size: 326 253
BsmBI CGTCTCn'nnnn_
Cuts at: 0 262 579
Size: 262 317
Bsp1286I G_dGCh'C
Cuts at: 0 326 473 579
Size: 326 147 106
Fragments arranged by size:
326 147 106
BsrDI GCAATG_nn'
Cuts at: 0 171 579
Size: 171 408
BsrFI r'CCGG_y
Cuts at: 0 104 117 579
Size: 104 13 462
Fragments arranged by size:
462 104 13
BssHII G'CGCG_C
Cuts at: 0 299 521 579
Size: 299 222 58
Fragments arranged by size:
299 222 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 228 312 372 579
Size: 228 84 60 207
Fragments arranged by size:
228 207 84 60
BtgI C'CryG_G
Cuts at: 0 284 390 453 579
Size: 284 106 63 126
Fragments arranged by size:
284 126 106 63
BtsI GCAGTG_nn'
Cuts at: 0 13 579
Size: 13 566
EaeI y'GGCC_r
Cuts at: 0 119 149 317 376 579
Size: 119 30 168 59 203
Fragments arranged by size:
203 168 119 59 30
EagI C'GGCC_G
Cuts at: 0 149 376 579
Size: 149 227 203
Fragments arranged by size:
227 203 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 84 477 579
Size: 84 393 102
Fragments arranged by size:
393 102 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 543 579
Size: 543 36
FspI TGC'GCA
Cuts at: 0 337 579
Size: 337 242
HaeII r_GCGC'y
Cuts at: 0 217 245 312 579
Size: 217 28 67 267
Fragments arranged by size:
267 217 67 28
KasI G'GCGC_C
Cuts at: 0 241 579
Size: 241 338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 579
Size: 479 100
MspA1I CmG'CkG
Cuts at: 0 148 392 455 509 563 579
Size: 148 244 63 54 54 16
Fragments arranged by size:
244 148 63 54 54 16
NaeI GCC'GGC
Cuts at: 0 106 579
Size: 106 473
NarI GG'CG_CC
Cuts at: 0 242 579
Size: 242 337
NgoMIV G'CCGG_C
Cuts at: 0 104 579
Size: 104 475
PmlI CAC'GTG
Cuts at: 0 228 579
Size: 228 351
PshAI GACnn'nnGTC
Cuts at: 0 78 579
Size: 78 501
PstI C_TGCA'G
Cuts at: 0 103 308 579
Size: 103 205 271
Fragments arranged by size:
271 205 103
PvuII CAG'CTG
Cuts at: 0 563 579
Size: 563 16
SacII CC_GC'GG
Cuts at: 0 393 456 579
Size: 393 63 123
Fragments arranged by size:
393 123 63
SfcI C'TryA_G
Cuts at: 0 99 304 579
Size: 99 205 275
Fragments arranged by size:
275 205 99
SfoI GGC'GCC
Cuts at: 0 243 579
Size: 243 336
SmaI CCC'GGG
Cuts at: 0 449 579
Size: 449 130
TatI w'GTAC_w
Cuts at: 0 290 579
Size: 290 289
XmaI C'CCGG_G
Cuts at: 0 447 579
Size: 447 132
Enzymes that do cut:
AlwNI AvaI BanI BanII BbeI BbvCI BglI BlpI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsiEI
BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BssHII BstAPI BtgI
BtsI EaeI EagI EciI Eco57MI FspI HaeII KasI
MmeI MspA1I NaeI NarI NgoMIV PmlI PshAI PstI
PvuII SacII SfcI SfoI SmaI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI
BmtI BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrGI BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EarI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmnI ZraI