Restriction map for
pFN21AE1678
(Linear) (Six-base) MAPSORT of: KIEE1678.seq Check: 1001 from: 1 to: 579
KIEE1678.orf
With 182 enzymes: SgfI *
July 15, 2010 18:44 ..
AfeI AGC'GCT
Cuts at: 0 118 579
Size: 118 461
AleI CACnn'nnGTG
Cuts at: 0 159 579
Size: 159 420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 439 579
Size: 439 140
AlwNI CAG_nnn'CTG
Cuts at: 0 141 471 579
Size: 141 330 108
Fragments arranged by size:
330 141 108
ApaI G_GGCC'C
Cuts at: 0 235 579
Size: 235 344
AvaI C'yCGr_G
Cuts at: 0 148 485 579
Size: 148 337 94
Fragments arranged by size:
337 148 94
BanI G'GyrC_C
Cuts at: 0 365 579
Size: 365 214
BanII G_rGCy'C
Cuts at: 0 235 579
Size: 235 344
BbvCI CC'TCA_GC
Cuts at: 0 200 579
Size: 200 379
BciVI GTATCCnnnnn_n'
Cuts at: 0 301 579
Size: 301 278
BlpI GC'TnA_GC
Cuts at: 0 131 173 579
Size: 131 42 406
Fragments arranged by size:
406 131 42
Bme1580I G_kGCm'C
Cuts at: 0 235 579
Size: 235 344
BmrI ACTGGGnnnn_n'
Cuts at: 0 250 524 579
Size: 250 274 55
Fragments arranged by size:
274 250 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 579
Size: 345 234
Bpu10I CC'TnA_GC
Cuts at: 0 200 579
Size: 200 379
BsaI GGTCTCn'nnnn_
Cuts at: 0 541 579
Size: 541 38
BsaAI yAC'GTr
Cuts at: 0 289 579
Size: 289 290
BsaHI Gr'CG_yC
Cuts at: 0 475 579
Size: 475 104
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 336 579
Size: 22 314 243
Fragments arranged by size:
314 243 22
BseYI C'CCAG_C
Cuts at: 0 234 464 579
Size: 234 230 115
Fragments arranged by size:
234 230 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 579
Size: 486 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 436 579
Size: 436 143
Bsp1286I G_dGCh'C
Cuts at: 0 235 579
Size: 235 344
BsrBI CCG'CTC
Cuts at: 0 147 248 579
Size: 147 101 331
Fragments arranged by size:
331 147 101
BsrDI GCAATG_nn'
Cuts at: 0 455 579
Size: 455 124
BssSI C'ACGA_G
Cuts at: 0 100 579
Size: 100 479
BstAPI GCAn_nnn'nTGC
Cuts at: 0 141 570 579
Size: 141 429 9
Fragments arranged by size:
429 141 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 301 381 579
Size: 301 80 198
Fragments arranged by size:
301 198 80
BtgI C'CryG_G
Cuts at: 0 258 386 579
Size: 258 128 193
Fragments arranged by size:
258 193 128
BtsI GCAGTG_nn'
Cuts at: 0 571 579
Size: 571 8
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 430 465 579
Size: 430 35 114
Fragments arranged by size:
430 114 35
EaeI y'GGCC_r
Cuts at: 0 335 383 579
Size: 335 48 196
Fragments arranged by size:
335 196 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 579
Size: 345 234
EcoO109I rG'GnC_Cy
Cuts at: 0 38 196 207 231 579
Size: 38 158 11 24 348
Fragments arranged by size:
348 158 38 24 11
HaeII r_GCGC'y
Cuts at: 0 120 471 579
Size: 120 351 108
Fragments arranged by size:
351 120 108
MscI TGG'CCA
Cuts at: 0 337 579
Size: 337 242
MslI CAynn'nnrTG
Cuts at: 0 159 299 310 325 579
Size: 159 140 11 15 254
Fragments arranged by size:
254 159 140 15 11
MspA1I CmG'CkG
Cuts at: 0 141 183 579
Size: 141 42 396
Fragments arranged by size:
396 141 42
NcoI C'CATG_G
Cuts at: 0 258 579
Size: 258 321
PpuMI rG'GwC_Cy
Cuts at: 0 196 207 579
Size: 196 11 372
Fragments arranged by size:
372 196 11
PspOMI G'GGCC_C
Cuts at: 0 231 579
Size: 231 348
PspXI vC'TCGA_Gb
Cuts at: 0 148 579
Size: 148 431
PstI C_TGCA'G
Cuts at: 0 182 579
Size: 182 397
PvuII CAG'CTG
Cuts at: 0 141 183 579
Size: 141 42 396
Fragments arranged by size:
396 141 42
SfcI C'TryA_G
Cuts at: 0 178 579
Size: 178 401
SmlI C'TyrA_G
Cuts at: 0 148 579
Size: 148 431
SnaBI TAC'GTA
Cuts at: 0 289 579
Size: 289 290
StyI C'CwwG_G
Cuts at: 0 258 444 579
Size: 258 186 135
Fragments arranged by size:
258 186 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 35 149 558 579
Size: 35 114 409 21
Fragments arranged by size:
409 114 35 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 579
Size: 384 195
XhoI C'TCGA_G
Cuts at: 0 148 579
Size: 148 431
Enzymes that do cut:
AfeI AleI AlfI AlwNI ApaI AvaI BanI BanII
BbvCI BciVI BlpI Bme1580I BmrI BpmI Bpu10I BsaI
BsaAI BsaHI BseRI BseYI BsgI BsmBI Bsp1286I BsrBI
BsrDI BssSI BstAPI BstXI BtgI BtsI CspCI EaeI
Eco57MI EcoO109I HaeII MscI MslI MspA1I NcoI PpuMI
PspOMI PspXI PstI PvuII SfcI SmlI SnaBI StyI
TaqII XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BcgI BclI BfrBI
BglI BglII BmgBI BmtI BplI BpuEI BsaBI BsaWI
BsaXI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI
BsrFI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XmaI XmnI
ZraI