(Linear) (Six-base) MAPSORT of: KIEE1676.seq  Check: 5293  from: 1  to: 570



KIEE1676.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

Acc65I G'GTAC_C

Cuts at:      0      77     570 
   Size:         77     493
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     570 
   Size:        535      35
 
AflII C'TTAA_G

Cuts at:      0     175     570 
   Size:        175     395
 
ApoI r'AATT_y

Cuts at:      0     286     469     484     509     570 
   Size:        286     183      15      25      61

  Fragments arranged by size: 

                 286     183      61      25      15
 
AvaI C'yCGr_G

Cuts at:      0     133     570 
   Size:        133     437
 
BanI G'GyrC_C

Cuts at:      0      77     126     570 
   Size:         77      49     444

  Fragments arranged by size: 

                 444      77      49
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     523     570 
   Size:        523      47
 
BclI T'GATC_A

Cuts at:      0     554     570 
   Size:        554      16
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     390     570 
   Size:        235     155     180

  Fragments arranged by size: 

                 235     180     155
 
Bpu10I CC'TnA_GC

Cuts at:      0      34     364     570 
   Size:         34     330     206

  Fragments arranged by size: 

                 330     206      34
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     570 
   Size:         37     533
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     151     570 
   Size:        109      42     419

  Fragments arranged by size: 

                 419     109      42
 
BsmI GAATG_Cn'

Cuts at:      0      54     220     570 
   Size:         54     166     350

  Fragments arranged by size: 

                 350     166      54
 
BspHI T'CATG_A

Cuts at:      0     326     570 
   Size:        326     244
 
BsrBI CCG'CTC

Cuts at:      0      38     570 
   Size:         38     532
 
BtgI C'CryG_G

Cuts at:      0      72     570 
   Size:         72     498
 
EarI CTCTTCn'nnn_

Cuts at:      0     289     570 
   Size:        289     281
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     133     570 
   Size:        133     437
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     390     535     570 
   Size:        235     155     145      35

  Fragments arranged by size: 

                 235     155     145      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     360     570 
   Size:        360     210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     370     402     570 
   Size:        370      32     168

  Fragments arranged by size: 

                 370     168      32
 
HincII GTy'rAC

Cuts at:      0     504     570 
   Size:        504      66
 
KpnI G_GTAC'C

Cuts at:      0      81     570 
   Size:         81     489
 
MspA1I CmG'CkG

Cuts at:      0     213     341     570 
   Size:        213     128     229

  Fragments arranged by size: 

                 229     213     128
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     290     570 
   Size:        290     280
 
PstI C_TGCA'G

Cuts at:      0     195     570 
   Size:        195     375
 
PvuII CAG'CTG

Cuts at:      0     213     570 
   Size:        213     357
 
SapI GCTCTTCn'nnn_

Cuts at:      0     289     570 
   Size:        289     281
 
SfcI C'TryA_G

Cuts at:      0     191     570 
   Size:        191     379
 
SmlI C'TyrA_G

Cuts at:      0      16     175     570 
   Size:         16     159     395

  Fragments arranged by size: 

                 395     159      16
 
Tth111I GACn'n_nGTC

Cuts at:      0     522     570 
   Size:        522      48
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     435     570 
   Size:        435     135
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII     ApoI     AvaI     BanI     BbsI     BclI
     BpmI   Bpu10I    BpuEI    BseRI     BsmI    BspHI    BsrBI     BtgI
     EarI     EciI  Eco57MI EcoO109I    Hin4I   HincII     KpnI   MspA1I
    PflMI     PstI    PvuII     SapI     SfcI     SmlI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI     XbaI
     XhoI     XmaI     XmnI     ZraI