(Linear) (Six-base) MAPSORT of: KIEE1675.seq  Check: 6864  from: 1  to: 567



KIEE1675.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:52  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     255     567 
   Size:        178      77     312

  Fragments arranged by size: 

                 312     178      77
 
AhdI GACnn_n'nnGTC

Cuts at:      0     357     567 
   Size:        357     210
 
AlwNI CAG_nnn'CTG

Cuts at:      0     345     567 
   Size:        345     222
 
ApoI r'AATT_y

Cuts at:      0     178     319     567 
   Size:        178     141     248

  Fragments arranged by size: 

                 248     178     141
 
BlpI GC'TnA_GC

Cuts at:      0      40     567 
   Size:         40     527
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     567 
   Size:        376     191
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     567 
   Size:        206     361
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     298     567 
   Size:        298     269
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     397     427     567 
   Size:        397      30     140

  Fragments arranged by size: 

                 397     140      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     205     567 
   Size:        205     362
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     567 
   Size:         41     526
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     567 
   Size:         41     526
 
BspHI T'CATG_A

Cuts at:      0     512     567 
   Size:        512      55
 
BstYI r'GATC_y

Cuts at:      0     517     567 
   Size:        517      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     274     550     567 
   Size:        274     276      17

  Fragments arranged by size: 

                 276     274      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     255     376     567 
   Size:        178      77     121     191

  Fragments arranged by size: 

                 191     178     121      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     567 
   Size:        107     460
 
EcoRI G'AATT_C

Cuts at:      0     178     319     567 
   Size:        178     141     248

  Fragments arranged by size: 

                 248     178     141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375     378     410     494     526     567 
   Size:        343      32       3      32      84      32      41

  Fragments arranged by size: 

                 343      84      41      32      32      32       3
 
MspA1I CmG'CkG

Cuts at:      0     345     567 
   Size:        345     222
 
PvuII CAG'CTG

Cuts at:      0     345     567 
   Size:        345     222
 
SmlI C'TyrA_G

Cuts at:      0     221     567 
   Size:        221     346
 
XbaI T'CTAG_A

Cuts at:      0     312     567 
   Size:        312     255
 
XmnI GAAnn'nnTTC

Cuts at:      0     436     567 
   Size:        436     131
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     ApoI     BlpI     BpmI    BpuEI     BsaI
    BsaXI    BseRI  BsiHKAI Bsp1286I    BspHI    BstYI     EarI  Eco57MI
 EcoO109I    EcoRI    Hin4I   MspA1I    PvuII     SmlI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     ZraI