(Linear) (Six-base) MAPSORT of: KIEE1672.seq  Check: 3627  from: 1  to: 567



KIEE1672.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     178     242     255     567 
   Size:        159      19      64      13     312

  Fragments arranged by size: 

                 312     159      64      19      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0     345     567 
   Size:        345     222
 
BlpI GC'TnA_GC

Cuts at:      0      40     567 
   Size:         40     527
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     158     567 
   Size:        158     409
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     147     205     567 
   Size:        147      58     362

  Fragments arranged by size: 

                 362     147      58
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     567 
   Size:         41     526
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     567 
   Size:         41     526
 
BspHI T'CATG_A

Cuts at:      0     512     567 
   Size:        512      55
 
BstYI r'GATC_y

Cuts at:      0     517     567 
   Size:        517      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     274     531     567 
   Size:        274     257      36

  Fragments arranged by size: 

                 274     257      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     178     242     255     567 
   Size:        159      19      64      13     312

  Fragments arranged by size: 

                 312     159      64      19      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     241     567 
   Size:        241     326
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     494     526     567 
   Size:        494      32      41

  Fragments arranged by size: 

                 494      41      32
 
MspA1I CmG'CkG

Cuts at:      0      44     345     567 
   Size:         44     301     222

  Fragments arranged by size: 

                 301     222      44
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      93     567 
   Size:         93     474
 
PvuII CAG'CTG

Cuts at:      0      44     345     567 
   Size:         44     301     222

  Fragments arranged by size: 

                 301     222      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     372     567 
   Size:        372     195
 
TatI w'GTAC_w

Cuts at:      0     473     567 
   Size:        473      94
 
XbaI T'CTAG_A

Cuts at:      0     312     567 
   Size:        312     255
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     117     567 
   Size:        117     450
 

 Enzymes that do cut:

     AcuI    AlwNI     BlpI     BplI    BseRI  BsiHKAI Bsp1286I    BspHI
    BstYI     EarI  Eco57MI    EcoNI    Hin4I   MspA1I    PflMI    PvuII
    TaqII     TatI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XhoI     XmaI     XmnI     ZraI