(Linear) (Six-base) MAPSORT of: KIEE1671.seq  Check: 4330  from: 1  to: 567



KIEE1671.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     255     567 
   Size:        178      77     312

  Fragments arranged by size: 

                 312     178      77
 
AlwNI CAG_nnn'CTG

Cuts at:      0     345     567 
   Size:        345     222
 
ApoI r'AATT_y

Cuts at:      0     319     567 
   Size:        319     248
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     395     567 
   Size:        395     172
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     567 
   Size:        206     361
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     205     386     567 
   Size:        205     181     181

  Fragments arranged by size: 

                 205     181     181
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     567 
   Size:         41     526
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     567 
   Size:         41     526
 
BspHI T'CATG_A

Cuts at:      0     512     567 
   Size:        512      55
 
BstYI r'GATC_y

Cuts at:      0     517     567 
   Size:        517      50
 
Bsu36I CC'TnA_GG

Cuts at:      0     185     567 
   Size:        185     382
 
EarI CTCTTCn'nnn_

Cuts at:      0     274     567 
   Size:        274     293
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     255     567 
   Size:        178      77     312

  Fragments arranged by size: 

                 312     178      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     249     281     494     526     567 
   Size:        249      32     213      32      41

  Fragments arranged by size: 

                 249     213      41      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     360     567 
   Size:        360     207
 
MspA1I CmG'CkG

Cuts at:      0     345     567 
   Size:        345     222
 
PvuII CAG'CTG

Cuts at:      0     345     567 
   Size:        345     222
 
SmlI C'TyrA_G

Cuts at:      0     221     567 
   Size:        221     346
 
XbaI T'CTAG_A

Cuts at:      0     312     567 
   Size:        312     255
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BbsI    BpuEI    BseRI  BsiHKAI Bsp1286I
    BspHI    BstYI   Bsu36I     EarI  Eco57MI    Hin4I     MmeI   MspA1I
    PvuII     SmlI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI