(Linear) (Six-base) MAPSORT of: KIEE1670.seq  Check: 3605  from: 1  to: 564



KIEE1670.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     564 
   Size:        252     312
 
AlwNI CAG_nnn'CTG

Cuts at:      0     342     564 
   Size:        342     222
 
ApoI r'AATT_y

Cuts at:      0     316     564 
   Size:        316     248
 
BglII A'GATC_T

Cuts at:      0     254     514     564 
   Size:        254     260      50

  Fragments arranged by size: 

                 260     254      50
 
BlpI GC'TnA_GC

Cuts at:      0      40     564 
   Size:         40     524
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     158     564 
   Size:        158     406
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     295     564 
   Size:        295     269
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     375     405     564 
   Size:        375      30     159

  Fragments arranged by size: 

                 375     159      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     147     205     564 
   Size:        147      58     359

  Fragments arranged by size: 

                 359     147      58
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     564 
   Size:         41     523
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     564 
   Size:         41     523
 
BspHI T'CATG_A

Cuts at:      0     509     564 
   Size:        509      55
 
BstYI r'GATC_y

Cuts at:      0     254     514     564 
   Size:        254     260      50

  Fragments arranged by size: 

                 260     254      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     271     564 
   Size:        271     293
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     564 
   Size:        252     312
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     400     564 
   Size:        400     164
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     564 
   Size:        107     457
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     246     278     375     407     491     523     564 
   Size:        246      32      97      32      84      32      41

  Fragments arranged by size: 

                 246      97      84      41      32      32      32
 
HincII GTy'rAC

Cuts at:      0     530     564 
   Size:        530      34
 
MspA1I CmG'CkG

Cuts at:      0      44     342     564 
   Size:         44     298     222

  Fragments arranged by size: 

                 298     222      44
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      93     564 
   Size:         93     471
 
PpuMI rG'GwC_Cy

Cuts at:      0     107     564 
   Size:        107     457
 
PvuII CAG'CTG

Cuts at:      0      44     342     564 
   Size:         44     298     222

  Fragments arranged by size: 

                 298     222      44
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     193     564 
   Size:        193     371
 
XmnI GAAnn'nnTTC

Cuts at:      0     433     564 
   Size:        433     131
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BglII     BlpI     BplI     BsaI    BsaXI
    BseRI  BsiHKAI Bsp1286I    BspHI    BstYI     EarI  Eco57MI    EcoNI
 EcoO109I    Hin4I   HincII   MspA1I    PflMI    PpuMI    PvuII     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
 Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI