(Linear) (Six-base) MAPSORT of: KIEE1669.seq  Check: 7467  from: 1  to: 564



KIEE1669.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     564 
   Size:        120     444
 
AlwNI CAG_nnn'CTG

Cuts at:      0     242     564 
   Size:        242     322
 
ApoI r'AATT_y

Cuts at:      0     103     564 
   Size:        103     461
 
AvaI C'yCGr_G

Cuts at:      0     119     564 
   Size:        119     445
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     179     564 
   Size:        179     385
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     118     206     564 
   Size:        118      88     358

  Fragments arranged by size: 

                 358     118      88
 
Bme1580I G_kGCm'C

Cuts at:      0     356     564 
   Size:        356     208
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      82     564 
   Size:         82     482
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     473     564 
   Size:        473      91
 
BsaAI yAC'GTr

Cuts at:      0     511     564 
   Size:        511      53
 
BsaBI GATnn'nnATC

Cuts at:      0      43     564 
   Size:         43     521
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     178     564 
   Size:        178     386
 
BseYI C'CCAG_C

Cuts at:      0      90     191     270     564 
   Size:         90     101      79     294

  Fragments arranged by size: 

                 294     101      90      79
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     564 
   Size:        491      73
 
BsiHKAI G_wGCw'C

Cuts at:      0     323     470     564 
   Size:        323     147      94

  Fragments arranged by size: 

                 323     147      94
 
Bsp1286I G_dGCh'C

Cuts at:      0     323     356     470     564 
   Size:        323      33     114      94

  Fragments arranged by size: 

                 323     114      94      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     250     271     564 
   Size:        250      21     293

  Fragments arranged by size: 

                 293     250      21
 
BssSI C'ACGA_G

Cuts at:      0     472     564 
   Size:        472      92
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     327     564 
   Size:        327     237
 
Bsu36I CC'TnA_GG

Cuts at:      0     425     564 
   Size:        425     139
 
BtgI C'CryG_G

Cuts at:      0     206     564 
   Size:        206     358
 
DraIII CAC_nnn'GTG

Cuts at:      0     327     474     564 
   Size:        327     147      90

  Fragments arranged by size: 

                 327     147      90
 
EaeI y'GGCC_r

Cuts at:      0      50     564 
   Size:         50     514
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     473     564 
   Size:        120     353      91

  Fragments arranged by size: 

                 353     120      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0     275     564 
   Size:        275     289
 
FspI TGC'GCA

Cuts at:      0     331     564 
   Size:        331     233
 
MscI TGG'CCA

Cuts at:      0      52     564 
   Size:         52     512
 
MslI CAynn'nnrTG

Cuts at:      0     523     564 
   Size:        523      41
 
MspA1I CmG'CkG

Cuts at:      0     416     564 
   Size:        416     148
 
NcoI C'CATG_G

Cuts at:      0     206     564 
   Size:        206     358
 
NspI r_CATG'y

Cuts at:      0     270     564 
   Size:        270     294
 
PfoI T'CCnGG_A

Cuts at:      0     481     564 
   Size:        481      83
 
PpuMI rG'GwC_Cy

Cuts at:      0     275     564 
   Size:        275     289
 
PstI C_TGCA'G

Cuts at:      0      10     564 
   Size:         10     554
 
SfcI C'TryA_G

Cuts at:      0       6     564 
   Size:          6     558
 
SphI G_CATG'C

Cuts at:      0     270     564 
   Size:        270     294
 
StyI C'CwwG_G

Cuts at:      0     206     564 
   Size:        206     358
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     173     564 
   Size:        173     391
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI     BbsI    BciVI Bme1580I     BmrI
     BpmI    BsaAI    BsaBI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BssSI   BstAPI   Bsu36I     BtgI   DraIII     EaeI  Eco57MI
 EcoO109I     FspI     MscI     MslI   MspA1I     NcoI     NspI     PfoI
    PpuMI     PstI     SfcI     SphI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI