(Linear) (Six-base) MAPSORT of: KIEE1668.seq  Check: 7095  from: 1  to: 564



KIEE1668.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     564 
   Size:         61     503
 
AlwNI CAG_nnn'CTG

Cuts at:      0     354     564 
   Size:        354     210
 
BamHI G'GATC_C

Cuts at:      0     279     564 
   Size:        279     285
 
BanI G'GyrC_C

Cuts at:      0     242     564 
   Size:        242     322
 
BanII G_rGCy'C

Cuts at:      0     206     473     504     564 
   Size:        206     267      31      60

  Fragments arranged by size: 

                 267     206      60      31
 
BmgBI CAC'GTC

Cuts at:      0     106     564 
   Size:        106     458
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     184     564 
   Size:        157      27     380

  Fragments arranged by size: 

                 380     157      27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     200     202     307     535     564 
   Size:        200       2     105     228      29

  Fragments arranged by size: 

                 228     200     105      29       2
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     564 
   Size:        442     122
 
BsiHKAI G_wGCw'C

Cuts at:      0     206     473     544     564 
   Size:        206     267      71      20

  Fragments arranged by size: 

                 267     206      71      20
 
BsmI GAATG_Cn'

Cuts at:      0      56     564 
   Size:         56     508
 
Bsp1286I G_dGCh'C

Cuts at:      0     206     473     504     544     564 
   Size:        206     267      31      40      20

  Fragments arranged by size: 

                 267     206      40      31      20
 
BspHI T'CATG_A

Cuts at:      0     323     564 
   Size:        323     241
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     354     564 
   Size:        354     210
 
BstYI r'GATC_y

Cuts at:      0     256     279     564 
   Size:        256      23     285

  Fragments arranged by size: 

                 285     256      23
 
BtgI C'CryG_G

Cuts at:      0     505     564 
   Size:        505      59
 
BtsI GCAGTG_nn'

Cuts at:      0     425     564 
   Size:        425     139
 
EarI CTCTTCn'nnn_

Cuts at:      0     478     564 
   Size:        478      86
 
EcoICRI GAG'CTC

Cuts at:      0     204     471     564 
   Size:        204     267      93

  Fragments arranged by size: 

                 267     204      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     157     184     564 
   Size:         61      96      27     380

  Fragments arranged by size: 

                 380      96      61      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15     426     564 
   Size:         15     411     138

  Fragments arranged by size: 

                 411     138      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     248     280     564 
   Size:        248      32     284

  Fragments arranged by size: 

                 284     248      32
 
MspA1I CmG'CkG

Cuts at:      0     351     564 
   Size:        351     213
 
PfoI T'CCnGG_A

Cuts at:      0     266     564 
   Size:        266     298
 
PpuMI rG'GwC_Cy

Cuts at:      0      15     564 
   Size:         15     549
 
PstI C_TGCA'G

Cuts at:      0     350     359     564 
   Size:        350       9     205

  Fragments arranged by size: 

                 350     205       9
 
SacI G_AGCT'C

Cuts at:      0     206     473     564 
   Size:        206     267      91

  Fragments arranged by size: 

                 267     206      91
 
SapI GCTCTTCn'nnn_

Cuts at:      0     478     564 
   Size:        478      86
 
SexAI A'CCwGG_T

Cuts at:      0     361     564 
   Size:        361     203
 
SfcI C'TryA_G

Cuts at:      0     346     355     564 
   Size:        346       9     209

  Fragments arranged by size: 

                 346     209       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     212     375     564 
   Size:        212     163     189

  Fragments arranged by size: 

                 212     189     163
 
Tth111I GACn'n_nGTC

Cuts at:      0     319     564 
   Size:        319     245
 
XmnI GAAnn'nnTTC

Cuts at:      0     439     564 
   Size:        439     125
 

 Enzymes that do cut:

     AcuI    AlwNI    BamHI     BanI    BanII    BmgBI     BpmI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI   BstAPI    BstYI     BtgI
     BtsI     EarI  EcoICRI  Eco57MI EcoO109I    Hin4I   MspA1I     PfoI
    PpuMI     PstI     SacI     SapI    SexAI     SfcI    TaqII  Tth111I
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI     XbaI     XcmI
     XhoI     XmaI     ZraI