Restriction map for
pFN21AE1666
(Linear) (Six-base) MAPSORT of: KIEE1666.seq Check: 7301 from: 1 to: 555
KIEE1666.orf
With 182 enzymes: SgfI *
July 15, 2010 18:44 ..
AatII G_ACGT'C
Cuts at: 0 277 555
Size: 277 278
AfeI AGC'GCT
Cuts at: 0 157 555
Size: 157 398
AleI CACnn'nnGTG
Cuts at: 0 142 478 555
Size: 142 336 77
Fragments arranged by size:
336 142 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 44 76 410 555
Size: 44 32 334 145
Fragments arranged by size:
334 145 44 32
BanI G'GyrC_C
Cuts at: 0 110 313 555
Size: 110 203 242
Fragments arranged by size:
242 203 110
BanII G_rGCy'C
Cuts at: 0 382 448 555
Size: 382 66 107
Fragments arranged by size:
382 107 66
BbeI G_GCGC'C
Cuts at: 0 114 317 555
Size: 114 203 238
Fragments arranged by size:
238 203 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 447 555
Size: 447 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 45 79 555
Size: 45 34 476
Fragments arranged by size:
476 45 34
BlpI GC'TnA_GC
Cuts at: 0 46 555
Size: 46 509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 153 425 555
Size: 124 29 272 130
Fragments arranged by size:
272 130 124 29
BsaHI Gr'CG_yC
Cuts at: 0 111 274 314 555
Size: 111 163 40 241
Fragments arranged by size:
241 163 111 40
BsaWI w'CCGG_w
Cuts at: 0 82 555
Size: 82 473
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 547 555
Size: 547 8
BseYI C'CCAG_C
Cuts at: 0 381 465 555
Size: 381 84 90
Fragments arranged by size:
381 90 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 555
Size: 107 448
BsiEI CG_ry'CG
Cuts at: 0 452 555
Size: 452 103
BsiHKAI G_wGCw'C
Cuts at: 0 139 362 555
Size: 139 223 193
Fragments arranged by size:
223 193 139
BsiWI C'GTAC_G
Cuts at: 0 201 555
Size: 201 354
Bsp1286I G_dGCh'C
Cuts at: 0 139 362 382 448 555
Size: 139 223 20 66 107
Fragments arranged by size:
223 139 107 66 20
BsrBI CCG'CTC
Cuts at: 0 50 108 555
Size: 50 58 447
Fragments arranged by size:
447 58 50
BstXI CCAn_nnnn'nTGG
Cuts at: 0 480 555
Size: 480 75
EaeI y'GGCC_r
Cuts at: 0 366 449 555
Size: 366 83 106
Fragments arranged by size:
366 106 83
EagI C'GGCC_G
Cuts at: 0 449 555
Size: 449 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 52 555
Size: 52 503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 153 425 555
Size: 124 29 272 130
Fragments arranged by size:
272 130 124 29
FspI TGC'GCA
Cuts at: 0 337 555
Size: 337 218
HaeII r_GCGC'y
Cuts at: 0 114 159 198 317 555
Size: 114 45 39 119 238
Fragments arranged by size:
238 119 114 45 39
HindIII A'AGCT_T
Cuts at: 0 352 555
Size: 352 203
KasI G'GCGC_C
Cuts at: 0 110 313 555
Size: 110 203 242
Fragments arranged by size:
242 203 110
MslI CAynn'nnrTG
Cuts at: 0 142 392 478 555
Size: 142 250 86 77
Fragments arranged by size:
250 142 86 77
NarI GG'CG_CC
Cuts at: 0 111 314 555
Size: 111 203 241
Fragments arranged by size:
241 203 111
PstI C_TGCA'G
Cuts at: 0 542 555
Size: 542 13
ScaI AGT'ACT
Cuts at: 0 217 555
Size: 217 338
SfcI C'TryA_G
Cuts at: 0 538 555
Size: 538 17
SfoI GGC'GCC
Cuts at: 0 112 315 555
Size: 112 203 240
Fragments arranged by size:
240 203 112
StyI C'CwwG_G
Cuts at: 0 341 555
Size: 341 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 450 555
Size: 450 105
TatI w'GTAC_w
Cuts at: 0 215 260 555
Size: 215 45 295
Fragments arranged by size:
295 215 45
ZraI GAC'GTC
Cuts at: 0 275 555
Size: 275 280
Enzymes that do cut:
AatII AfeI AleI AlfI BanI BanII BbeI BbsI
BcgI BlpI BpmI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BsrBI BstXI EaeI EagI
EciI Eco57MI FspI HaeII HindIII KasI MslI NarI
PstI ScaI SfcI SfoI StyI TaqII TatI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HpaI KpnI MfeI MluI MmeI MscI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI