(Linear) (Six-base) MAPSORT of: KIEE1664.seq  Check: 932  from: 1  to: 549



KIEE1664.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     549 
   Size:         40     509
 
AfeI AGC'GCT

Cuts at:      0     496     538     549 
   Size:        496      42      11

  Fragments arranged by size: 

                 496      42      11
 
AflIII A'CryG_T

Cuts at:      0     136     549 
   Size:        136     413
 
AvaI C'yCGr_G

Cuts at:      0     316     421     549 
   Size:        316     105     128

  Fragments arranged by size: 

                 316     128     105
 
BamHI G'GATC_C

Cuts at:      0      98     549 
   Size:         98     451
 
BanI G'GyrC_C

Cuts at:      0      55     549 
   Size:         55     494
 
BanII G_rGCy'C

Cuts at:      0     416     549 
   Size:        416     133
 
BclI T'GATC_A

Cuts at:      0       2     549 
   Size:          2     547
 
BmgBI CAC'GTC

Cuts at:      0     137     549 
   Size:        137     412
 
BsaHI Gr'CG_yC

Cuts at:      0      79     549 
   Size:         79     470
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28      58     505     535     549 
   Size:         28      30     447      30      14

  Fragments arranged by size: 

                 447      30      30      28      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52      55     160     549 
   Size:         52       3     105     389

  Fragments arranged by size: 

                 389     105      52       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     549 
   Size:        511      38
 
Bsp1286I G_dGCh'C

Cuts at:      0     416     549 
   Size:        416     133
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     549 
   Size:        134     415
 
BsrFI r'CCGG_y

Cuts at:      0     467     549 
   Size:        467      82
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     329     489     549 
   Size:        329     160      60

  Fragments arranged by size: 

                 329     160      60
 
BstEII G'GTnAC_C

Cuts at:      0     253     549 
   Size:        253     296
 
BstYI r'GATC_y

Cuts at:      0      98     156     549 
   Size:         98      58     393

  Fragments arranged by size: 

                 393      98      58
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     513     548     549 
   Size:        513      35       1

  Fragments arranged by size: 

                 513      35       1
 
EarI CTCTTCn'nnn_

Cuts at:      0     409     549 
   Size:        409     140
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     549 
   Size:         40     509
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     346     549 
   Size:        346     203
 
EcoO109I rG'GnC_Cy

Cuts at:      0     426     453     549 
   Size:        426      27      96

  Fragments arranged by size: 

                 426      96      27
 
EcoRV GAT'ATC

Cuts at:      0     144     549 
   Size:        144     405
 
HaeII r_GCGC'y

Cuts at:      0     498     540     549 
   Size:        498      42       9

  Fragments arranged by size: 

                 498      42       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60     549 
   Size:         28      32     489

  Fragments arranged by size: 

                 489      32      28
 
MspA1I CmG'CkG

Cuts at:      0      93     549 
   Size:         93     456
 
PpuMI rG'GwC_Cy

Cuts at:      0     426     453     549 
   Size:        426      27      96

  Fragments arranged by size: 

                 426      96      27
 
PshAI GACnn'nnGTC

Cuts at:      0     292     549 
   Size:        292     257
 
PspXI vC'TCGA_Gb

Cuts at:      0     316     549 
   Size:        316     233
 
PstI C_TGCA'G

Cuts at:      0     398     461     549 
   Size:        398      63      88

  Fragments arranged by size: 

                 398      88      63
 
PvuII CAG'CTG

Cuts at:      0      93     549 
   Size:         93     456
 
SfcI C'TryA_G

Cuts at:      0     394     457     549 
   Size:        394      63      92

  Fragments arranged by size: 

                 394      92      63
 
SmlI C'TyrA_G

Cuts at:      0     316     549 
   Size:        316     233
 
StyI C'CwwG_G

Cuts at:      0      59     272     549 
   Size:         59     213     277

  Fragments arranged by size: 

                 277     213      59
 
XhoI C'TCGA_G

Cuts at:      0     316     549 
   Size:        316     233
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AvaI    BamHI     BanI    BanII     BclI
    BmgBI    BsaHI    BsaXI    BseRI     BsgI Bsp1286I    BspMI    BsrFI
   BstAPI   BstEII    BstYI    CspCI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRV    HaeII    Hin4I   MspA1I    PpuMI    PshAI    PspXI     PstI
    PvuII     SfcI     SmlI     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoRI     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI