Restriction map for
pFN21AE1661
(Linear) (Six-base) MAPSORT of: KIEE1661.seq Check: 6089 from: 1 to: 537
KIEE1661.orf
With 182 enzymes: SgfI *
July 15, 2010 18:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 270 537
Size: 270 267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 537
Size: 463 74
AfeI AGC'GCT
Cuts at: 0 262 537
Size: 262 275
AflIII A'CryG_T
Cuts at: 0 391 537
Size: 391 146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 537
Size: 187 350
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 537
Size: 220 32 285
Fragments arranged by size:
285 220 32
AlwNI CAG_nnn'CTG
Cuts at: 0 285 537
Size: 285 252
BanI G'GyrC_C
Cuts at: 0 354 537
Size: 354 183
BanII G_rGCy'C
Cuts at: 0 28 537
Size: 28 509
BlpI GC'TnA_GC
Cuts at: 0 373 385 537
Size: 373 12 152
Fragments arranged by size:
373 152 12
Bme1580I G_kGCm'C
Cuts at: 0 359 537
Size: 359 178
BmtI G_CTAG'C
Cuts at: 0 191 537
Size: 191 346
Bpu10I CC'TnA_GC
Cuts at: 0 179 537
Size: 179 358
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 537
Size: 220 30 287
Fragments arranged by size:
287 220 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 104 537
Size: 104 433
BsiHKAI G_wGCw'C
Cuts at: 0 28 537
Size: 28 509
BsmBI CGTCTCn'nnnn_
Cuts at: 0 223 537
Size: 223 314
Bsp1286I G_dGCh'C
Cuts at: 0 28 359 537
Size: 28 331 178
Fragments arranged by size:
331 178 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 270 422 537
Size: 270 152 115
Fragments arranged by size:
270 152 115
BtgI C'CryG_G
Cuts at: 0 240 537
Size: 240 297
BtsI GCAGTG_nn'
Cuts at: 0 114 537
Size: 114 423
EaeI y'GGCC_r
Cuts at: 0 2 47 365 537
Size: 2 45 318 172
Fragments arranged by size:
318 172 45 2
EcoICRI GAG'CTC
Cuts at: 0 26 537
Size: 26 511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 537
Size: 463 74
EcoO109I rG'GnC_Cy
Cuts at: 0 41 537
Size: 41 496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 153 537
Size: 153 384
HaeII r_GCGC'y
Cuts at: 0 264 537
Size: 264 273
HindIII A'AGCT_T
Cuts at: 0 460 537
Size: 460 77
MfeI C'AATT_G
Cuts at: 0 488 537
Size: 488 49
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 537
Size: 158 379
MscI TGG'CCA
Cuts at: 0 49 367 537
Size: 49 318 170
Fragments arranged by size:
318 170 49
MslI CAynn'nnrTG
Cuts at: 0 169 537
Size: 169 368
MspA1I CmG'CkG
Cuts at: 0 56 97 537
Size: 56 41 440
Fragments arranged by size:
440 56 41
NheI G'CTAG_C
Cuts at: 0 187 537
Size: 187 350
NspI r_CATG'y
Cuts at: 0 395 537
Size: 395 142
PciI A'CATG_T
Cuts at: 0 391 537
Size: 391 146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 537
Size: 220 32 285
Fragments arranged by size:
285 220 32
PpuMI rG'GwC_Cy
Cuts at: 0 41 537
Size: 41 496
PshAI GACnn'nnGTC
Cuts at: 0 215 537
Size: 215 322
PstI C_TGCA'G
Cuts at: 0 14 121 537
Size: 14 107 416
Fragments arranged by size:
416 107 14
PvuII CAG'CTG
Cuts at: 0 56 97 537
Size: 56 41 440
Fragments arranged by size:
440 56 41
SacI G_AGCT'C
Cuts at: 0 28 537
Size: 28 509
SanDI GG'GwC_CC
Cuts at: 0 41 537
Size: 41 496
SfcI C'TryA_G
Cuts at: 0 10 117 537
Size: 10 107 420
Fragments arranged by size:
420 107 10
XmnI GAAnn'nnTTC
Cuts at: 0 235 537
Size: 235 302
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AloI AlwNI BanI
BanII BlpI Bme1580I BmtI Bpu10I BsaXI BseRI BsiHKAI
BsmBI Bsp1286I BspMI BtgI BtsI EaeI EcoICRI Eco57MI
EcoO109I FalI HaeII HindIII MfeI MmeI MscI MslI
MspA1I NheI NspI PciI PpiI PpuMI PshAI PstI
PvuII SacI SanDI SfcI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HpaI KasI KpnI MluI NaeI NarI
NcoI NdeI NgoMIV NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI