(Linear) (Six-base) MAPSORT of: KIEE1659.seq  Check: 5654  from: 1  to: 528



KIEE1659.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6      94     100     528 
   Size:          6      88       6     428

  Fragments arranged by size: 

                 428      88       6       6
 
AflII C'TTAA_G

Cuts at:      0     192     528 
   Size:        192     336
 
AlwNI CAG_nnn'CTG

Cuts at:      0     435     528 
   Size:        435      93
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      82     528 
   Size:         82     446
 
BsaWI w'CCGG_w

Cuts at:      0     317     528 
   Size:        317     211
 
BspEI T'CCGG_A

Cuts at:      0     317     528 
   Size:        317     211
 
BsrDI GCAATG_nn'

Cuts at:      0     419     514     528 
   Size:        419      95      14

  Fragments arranged by size: 

                 419      95      14
 
Bsu36I CC'TnA_GG

Cuts at:      0     461     528 
   Size:        461      67
 
BtsI GCAGTG_nn'

Cuts at:      0     159     528 
   Size:        159     369
 
ClaI AT'CG_AT

Cuts at:      0     234     528 
   Size:        234     294
 
DraI TTT'AAA

Cuts at:      0     285     528 
   Size:        285     243
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6      94     100     528 
   Size:          6      88       6     428

  Fragments arranged by size: 

                 428      88       6       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     363     528 
   Size:        363     165
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     227     259     423     455     528 
   Size:        227      32     164      32      73

  Fragments arranged by size: 

                 227     164      73      32      32
 
NspI r_CATG'y

Cuts at:      0     395     528 
   Size:        395     133
 
PpuMI rG'GwC_Cy

Cuts at:      0     363     528 
   Size:        363     165
 
SmlI C'TyrA_G

Cuts at:      0     192     528 
   Size:        192     336
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      83     115     528 
   Size:         83      32     413

  Fragments arranged by size: 

                 413      83      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     204     528 
   Size:        204     324
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     BbsI    BsaWI    BspEI    BsrDI   Bsu36I
     BtsI     ClaI     DraI  Eco57MI EcoO109I    Hin4I     NspI    PpuMI
     SmlI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI