Restriction map for
pFN21AE1657
(Linear) (Six-base) MAPSORT of: KIEE1657.seq Check: 4989 from: 1 to: 519
KIEE1657.orf
With 182 enzymes: SgfI *
July 15, 2010 18:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 280 519
Size: 280 239
AflIII A'CryG_T
Cuts at: 0 184 519
Size: 184 335
AleI CACnn'nnGTG
Cuts at: 0 109 519
Size: 109 410
ApaI G_GGCC'C
Cuts at: 0 169 519
Size: 169 350
ApoI r'AATT_y
Cuts at: 0 418 519
Size: 418 101
AvaI C'yCGr_G
Cuts at: 0 168 454 519
Size: 168 286 65
Fragments arranged by size:
286 168 65
AvrII C'CTAG_G
Cuts at: 0 60 519
Size: 60 459
BanI G'GyrC_C
Cuts at: 0 6 519
Size: 6 513
BanII G_rGCy'C
Cuts at: 0 104 169 519
Size: 104 65 350
Fragments arranged by size:
350 104 65
BbeI G_GCGC'C
Cuts at: 0 10 519
Size: 10 509
BbsI GAAGACnn'nnnn_
Cuts at: 0 260 519
Size: 260 259
BbvCI CC'TCA_GC
Cuts at: 0 76 519
Size: 76 443
BlpI GC'TnA_GC
Cuts at: 0 470 519
Size: 470 49
Bme1580I G_kGCm'C
Cuts at: 0 169 519
Size: 169 350
BmgBI CAC'GTC
Cuts at: 0 123 519
Size: 123 396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 519
Size: 303 216
Bpu10I CC'TnA_GC
Cuts at: 0 76 519
Size: 76 443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 519
Size: 191 328
BsaAI yAC'GTr
Cuts at: 0 185 519
Size: 185 334
BsaHI Gr'CG_yC
Cuts at: 0 7 29 137 519
Size: 7 22 108 382
Fragments arranged by size:
382 108 22 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 519
Size: 79 30 410
Fragments arranged by size:
410 79 30
BseYI C'CCAG_C
Cuts at: 0 10 519
Size: 10 509
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 519
Size: 258 261
BsmI GAATG_Cn'
Cuts at: 0 144 460 519
Size: 144 316 59
Fragments arranged by size:
316 144 59
Bsp1286I G_dGCh'C
Cuts at: 0 104 169 519
Size: 104 65 350
Fragments arranged by size:
350 104 65
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 280 519
Size: 280 239
BsrDI GCAATG_nn'
Cuts at: 0 232 519
Size: 232 287
BsrFI r'CCGG_y
Cuts at: 0 81 519
Size: 81 438
BstXI CCAn_nnnn'nTGG
Cuts at: 0 111 519
Size: 111 408
BtgI C'CryG_G
Cuts at: 0 68 359 519
Size: 68 291 160
Fragments arranged by size:
291 160 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 488 519
Size: 488 31
BtsI GCAGTG_nn'
Cuts at: 0 446 519
Size: 446 73
EaeI y'GGCC_r
Cuts at: 0 494 519
Size: 494 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 100 519
Size: 100 419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 519
Size: 303 216
EcoO109I rG'GnC_Cy
Cuts at: 0 287 519
Size: 287 232
HaeII r_GCGC'y
Cuts at: 0 10 76 344 519
Size: 10 66 268 175
Fragments arranged by size:
268 175 66 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 519
Size: 79 32 408
Fragments arranged by size:
408 79 32
KasI G'GCGC_C
Cuts at: 0 6 519
Size: 6 513
MscI TGG'CCA
Cuts at: 0 496 519
Size: 496 23
MslI CAynn'nnrTG
Cuts at: 0 109 519
Size: 109 410
MspA1I CmG'CkG
Cuts at: 0 14 519
Size: 14 505
NaeI GCC'GGC
Cuts at: 0 83 519
Size: 83 436
NarI GG'CG_CC
Cuts at: 0 7 519
Size: 7 512
NcoI C'CATG_G
Cuts at: 0 359 519
Size: 359 160
NgoMIV G'CCGG_C
Cuts at: 0 81 519
Size: 81 438
PasI CC'CwG_GG
Cuts at: 0 291 519
Size: 291 228
PflMI CCAn_nnn'nTGG
Cuts at: 0 162 519
Size: 162 357
PspOMI G'GGCC_C
Cuts at: 0 165 519
Size: 165 354
SfoI GGC'GCC
Cuts at: 0 8 519
Size: 8 511
SmaI CCC'GGG
Cuts at: 0 170 456 519
Size: 170 286 63
Fragments arranged by size:
286 170 63
SmlI C'TyrA_G
Cuts at: 0 206 519
Size: 206 313
StyI C'CwwG_G
Cuts at: 0 60 359 497 519
Size: 60 299 138 22
Fragments arranged by size:
299 138 60 22
TatI w'GTAC_w
Cuts at: 0 308 519
Size: 308 211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 438 519
Size: 438 81
XmaI C'CCGG_G
Cuts at: 0 168 454 519
Size: 168 286 65
Fragments arranged by size:
286 168 65
Enzymes that do cut:
AarI AflIII AleI ApaI ApoI AvaI AvrII BanI
BanII BbeI BbsI BbvCI BlpI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseYI BsgI BsmI
Bsp1286I BspMI BsrDI BsrFI BstXI BtgI BtgZI BtsI
EaeI EciI Eco57MI EcoO109I HaeII Hin4I KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI
PspOMI SfoI SmaI SmlI StyI TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
BaeI BamHI BcgI BciVI BclI BfrBI BglI BglII
BmrI BmtI BplI BsaI BsaBI BsaWI BseRI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XhoI XmnI ZraI