(Linear) (Six-base) MAPSORT of: KIEE1656.seq  Check: 3412  from: 1  to: 519



KIEE1656.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     519 
   Size:        130     389
 
AleI CACnn'nnGTG

Cuts at:      0     133     193     519 
   Size:        133      60     326

  Fragments arranged by size: 

                 326     133      60
 
AlwNI CAG_nnn'CTG

Cuts at:      0     471     519 
   Size:        471      48
 
ApaLI G'TGCA_C

Cuts at:      0     304     519 
   Size:        304     215
 
BanI G'GyrC_C

Cuts at:      0     219     519 
   Size:        219     300
 
BanII G_rGCy'C

Cuts at:      0     333     519 
   Size:        333     186
 
BbeI G_GCGC'C

Cuts at:      0     223     519 
   Size:        223     296
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     251     519 
   Size:        251     268
 
BlpI GC'TnA_GC

Cuts at:      0      88     253     519 
   Size:         88     165     266

  Fragments arranged by size: 

                 266     165      88
 
Bme1580I G_kGCm'C

Cuts at:      0     308     519 
   Size:        308     211
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     317     466     519 
   Size:        193     124     149      53

  Fragments arranged by size: 

                 193     149     124      53
 
BsaHI Gr'CG_yC

Cuts at:      0     220     383     519 
   Size:        220     163     136

  Fragments arranged by size: 

                 220     163     136
 
BsiHKAI G_wGCw'C

Cuts at:      0     308     519 
   Size:        308     211
 
Bsp1286I G_dGCh'C

Cuts at:      0     308     333     519 
   Size:        308      25     186

  Fragments arranged by size: 

                 308     186      25
 
BsrBI CCG'CTC

Cuts at:      0     365     519 
   Size:        365     154
 
BsrFI r'CCGG_y

Cuts at:      0     455     519 
   Size:        455      64
 
BtgI C'CryG_G

Cuts at:      0     411     519 
   Size:        411     108
 
BtsI GCAGTG_nn'

Cuts at:      0     306     519 
   Size:        306     213
 
DraIII CAC_nnn'GTG

Cuts at:      0     312     519 
   Size:        312     207
 
EaeI y'GGCC_r

Cuts at:      0     202     519 
   Size:        202     317
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     388     519 
   Size:        388     131
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     193     317     466     519 
   Size:        130      63     124     149      53

  Fragments arranged by size: 

                 149     130     124      63      53
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     262     519 
   Size:        262     257
 
EcoO109I rG'GnC_Cy

Cuts at:      0     105     282     519 
   Size:        105     177     237

  Fragments arranged by size: 

                 237     177     105
 
HaeII r_GCGC'y

Cuts at:      0     223     349     411     519 
   Size:        223     126      62     108

  Fragments arranged by size: 

                 223     126     108      62
 
KasI G'GCGC_C

Cuts at:      0     219     519 
   Size:        219     300
 
MslI CAynn'nnrTG

Cuts at:      0     133     193     519 
   Size:        133      60     326

  Fragments arranged by size: 

                 326     133      60
 
MspA1I CmG'CkG

Cuts at:      0     171     293     413     519 
   Size:        171     122     120     106

  Fragments arranged by size: 

                 171     122     120     106
 
NarI GG'CG_CC

Cuts at:      0     220     519 
   Size:        220     299
 
NspI r_CATG'y

Cuts at:      0      51     519 
   Size:         51     468
 
PpuMI rG'GwC_Cy

Cuts at:      0     105     519 
   Size:        105     414
 
PvuII CAG'CTG

Cuts at:      0     293     519 
   Size:        293     226
 
SacII CC_GC'GG

Cuts at:      0     414     519 
   Size:        414     105
 
SfoI GGC'GCC

Cuts at:      0     221     519 
   Size:        221     298
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      79     519 
   Size:         79     440
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI    ApaLI     BanI    BanII     BbeI     BbsI
     BlpI Bme1580I     BpmI    BsaHI  BsiHKAI Bsp1286I    BsrBI    BsrFI
     BtgI     BtsI   DraIII     EaeI     EciI  Eco57MI    EcoNI EcoO109I
    HaeII     KasI     MslI   MspA1I     NarI     NspI    PpuMI    PvuII
    SacII     SfoI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI