(Linear) (Six-base) MAPSORT of: KIEE1652.seq  Check: 2639  from: 1  to: 507



KIEE1652.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     440     507 
   Size:         94     346      67

  Fragments arranged by size: 

                 346      94      67
 
AlwNI CAG_nnn'CTG

Cuts at:      0     163     507 
   Size:        163     344
 
AseI AT'TA_AT

Cuts at:      0      80     507 
   Size:         80     427
 
AvaI C'yCGr_G

Cuts at:      0     331     507 
   Size:        331     176
 
BamHI G'GATC_C

Cuts at:      0     216     507 
   Size:        216     291
 
BanII G_rGCy'C

Cuts at:      0     338     507 
   Size:        338     169
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     197     507 
   Size:        197     310
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      80     151     507 
   Size:         80      71     356

  Fragments arranged by size: 

                 356      80      71
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      26     507 
   Size:         26     481
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     409     507 
   Size:        220     189      98

  Fragments arranged by size: 

                 220     189      98
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     428     507 
   Size:        428      79
 
BsaBI GATnn'nnATC

Cuts at:      0      89     507 
   Size:         89     418
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     414     507 
   Size:        414      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      11     507 
   Size:         11     496
 
Bsp1286I G_dGCh'C

Cuts at:      0     338     507 
   Size:        338     169
 
BsrDI GCAATG_nn'

Cuts at:      0     286     416     507 
   Size:        286     130      91

  Fragments arranged by size: 

                 286     130      91
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     228     507 
   Size:        228     279
 
BstYI r'GATC_y

Cuts at:      0     216     315     507 
   Size:        216      99     192

  Fragments arranged by size: 

                 216     192      99
 
BtsI GCAGTG_nn'

Cuts at:      0     479     507 
   Size:        479      28
 
DraIII CAC_nnn'GTG

Cuts at:      0     142     507 
   Size:        142     365
 
EaeI y'GGCC_r

Cuts at:      0     324     507 
   Size:        324     183
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     440     507 
   Size:         94     346      67

  Fragments arranged by size: 

                 346      94      67
 
EcoO109I rG'GnC_Cy

Cuts at:      0     377     507 
   Size:        377     130
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     300     332     507 
   Size:        300      32     175

  Fragments arranged by size: 

                 300     175      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     507 
   Size:        252     255
 
MscI TGG'CCA

Cuts at:      0     326     507 
   Size:        326     181
 
NspI r_CATG'y

Cuts at:      0     182     507 
   Size:        182     325
 
PfoI T'CCnGG_A

Cuts at:      0      12     404     507 
   Size:         12     392     103

  Fragments arranged by size: 

                 392     103      12
 
PpuMI rG'GwC_Cy

Cuts at:      0     377     507 
   Size:        377     130
 
PshAI GACnn'nnGTC

Cuts at:      0     157     507 
   Size:        157     350
 
SmlI C'TyrA_G

Cuts at:      0     199     388     507 
   Size:        199     189     119

  Fragments arranged by size: 

                 199     189     119
 
XmnI GAAnn'nnTTC

Cuts at:      0     346     507 
   Size:        346     161
 

 Enzymes that do cut:

     AcuI    AlwNI     AseI     AvaI    BamHI    BanII    BciVI     BmrI
     BplI    BpuEI     BsaI    BsaBI    BseRI    BsmBI Bsp1286I    BsrDI
    BstXI    BstYI     BtsI   DraIII     EaeI  Eco57MI EcoO109I    Hin4I
     MmeI     MscI     NspI     PfoI    PpuMI    PshAI     SmlI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI    AsiSI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI