(Linear) (Six-base) MAPSORT of: KIEE1650.seq  Check: 1774  from: 1  to: 492



KIEE1650.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AatII G_ACGT'C

Cuts at:      0      28     492 
   Size:         28     464
 
AflIII A'CryG_T

Cuts at:      0      74     492 
   Size:         74     418
 
BanII G_rGCy'C

Cuts at:      0     477     492 
   Size:        477      15
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      12      46     429     463     492 
   Size:         12      34     383      34      29

  Fragments arranged by size: 

                 383      34      34      29      12
 
BglI GCCn_nnn'nGGC

Cuts at:      0      56     265     481     492 
   Size:         56     209     216      11

  Fragments arranged by size: 

                 216     209      56      11
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     492 
   Size:        491       1
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     492 
   Size:        259     233
 
BsaHI Gr'CG_yC

Cuts at:      0      25     227     492 
   Size:         25     202     265

  Fragments arranged by size: 

                 265     202      25
 
BseYI C'CCAG_C

Cuts at:      0     230     255     492 
   Size:        230      25     237

  Fragments arranged by size: 

                 237     230      25
 
BsiHKAI G_wGCw'C

Cuts at:      0      35     188     329     492 
   Size:         35     153     141     163

  Fragments arranged by size: 

                 163     153     141      35
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     188     329     477     492 
   Size:         35     153     141     148      15

  Fragments arranged by size: 

                 153     148     141      35      15
 
BsrGI T'GTAC_A

Cuts at:      0     111     492 
   Size:        111     381
 
BssSI C'ACGA_G

Cuts at:      0     187     492 
   Size:        187     305
 
EarI CTCTTCn'nnn_

Cuts at:      0     167     492 
   Size:        167     325
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     492 
   Size:        491       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16     492 
   Size:         16     476
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     286     318     405     437     492 
   Size:        286      32      87      32      55

  Fragments arranged by size: 

                 286      87      55      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     373     492 
   Size:        373     119
 
SmlI C'TyrA_G

Cuts at:      0     274     492 
   Size:        274     218
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     403     492 
   Size:        403      89
 
TatI w'GTAC_w

Cuts at:      0     111     492 
   Size:        111     381
 
ZraI GAC'GTC

Cuts at:      0      26     492 
   Size:         26     466
 

 Enzymes that do cut:

    AatII   AflIII    BanII     BcgI     BglI     BpmI    BpuEI    BsaHI
    BseYI  BsiHKAI Bsp1286I    BsrGI    BssSI     EarI  Eco57MI EcoO109I
    Hin4I     MslI     SmlI    TaqII     TatI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI