(Linear) (Six-base) MAPSORT of: KIEE1646.seq  Check: 8667  from: 1  to: 483



KIEE1646.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AccI GT'mk_AC

Cuts at:      0     158     483 
   Size:        158     325
 
AvrII C'CTAG_G

Cuts at:      0     303     483 
   Size:        303     180
 
BsaWI w'CCGG_w

Cuts at:      0     133     483 
   Size:        133     350
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     132     483 
   Size:        132     351
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     212     325     483 
   Size:         95     117     113     158

  Fragments arranged by size: 

                 158     117     113      95
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     212     325     483 
   Size:         95     117     113     158

  Fragments arranged by size: 

                 158     117     113      95
 
BsrBI CCG'CTC

Cuts at:      0     153     483 
   Size:        153     330
 
BsrDI GCAATG_nn'

Cuts at:      0      27     483 
   Size:         27     456
 
BstEII G'GTnAC_C

Cuts at:      0     298     483 
   Size:        298     185
 
BtsI GCAGTG_nn'

Cuts at:      0     161     483 
   Size:        161     322
 
EaeI y'GGCC_r

Cuts at:      0      35     483 
   Size:         35     448
 
EarI CTCTTCn'nnn_

Cuts at:      0     364     483 
   Size:        364     119
 
EcoO109I rG'GnC_Cy

Cuts at:      0     104     483 
   Size:        104     379
 
MscI TGG'CCA

Cuts at:      0      37     483 
   Size:         37     446
 
MslI CAynn'nnrTG

Cuts at:      0      55     483 
   Size:         55     428
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      87     483 
   Size:         87     396
 
PpuMI rG'GwC_Cy

Cuts at:      0     104     483 
   Size:        104     379
 
ScaI AGT'ACT

Cuts at:      0     289     483 
   Size:        289     194
 
SfcI C'TryA_G

Cuts at:      0      14     483 
   Size:         14     469
 
StyI C'CwwG_G

Cuts at:      0     303     483 
   Size:        303     180
 
TatI w'GTAC_w

Cuts at:      0     266     287     483 
   Size:        266      21     196

  Fragments arranged by size: 

                 266     196      21
 
XmnI GAAnn'nnTTC

Cuts at:      0      70     483 
   Size:         70     413
 

 Enzymes that do cut:

     AccI    AvrII    BsaWI    BseRI  BsiHKAI Bsp1286I    BsrBI    BsrDI
   BstEII     BtsI     EaeI     EarI EcoO109I     MscI     MslI    PflMI
    PpuMI     ScaI     SfcI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI