(Linear) (Six-base) MAPSORT of: KIEE1643.seq  Check: 9026  from: 1  to: 474



KIEE1643.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     280     416     474 
   Size:         58     222     136      58

  Fragments arranged by size: 

                 222     136      58      58
 
AflIII A'CryG_T

Cuts at:      0     150     474 
   Size:        150     324
 
AhdI GACnn_n'nnGTC

Cuts at:      0     114     474 
   Size:        114     360
 
AvaI C'yCGr_G

Cuts at:      0      99     295     474 
   Size:         99     196     179

  Fragments arranged by size: 

                 196     179      99
 
BbvCI CC'TCA_GC

Cuts at:      0      29     474 
   Size:         29     445
 
BglI GCCn_nnn'nGGC

Cuts at:      0     335     474 
   Size:        335     139
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     439     474 
   Size:        439      35
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     474 
   Size:        104     370
 
Bpu10I CC'TnA_GC

Cuts at:      0      29     474 
   Size:         29     445
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     474 
   Size:        184     290
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     151     474 
   Size:        151     323
 
BsaBI GATnn'nnATC

Cuts at:      0      53     474 
   Size:         53     421
 
BsmI GAATG_Cn'

Cuts at:      0      41     474 
   Size:         41     433
 
BsrGI T'GTAC_A

Cuts at:      0     284     474 
   Size:        284     190
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     192     474 
   Size:        192     282
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     366     474 
   Size:        366     108
 
BtsI GCAGTG_nn'

Cuts at:      0     250     474 
   Size:        250     224
 
EarI CTCTTCn'nnn_

Cuts at:      0     322     474 
   Size:        322     152
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     104     280     416     474 
   Size:         58      46     176     136      58

  Fragments arranged by size: 

                 176     136      58      58      46
 
EcoRV GAT'ATC

Cuts at:      0      51     474 
   Size:         51     423
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     100     132     197     229     474 
   Size:        100      32      65      32     245

  Fragments arranged by size: 

                 245     100      65      32      32
 
HincII GTy'rAC

Cuts at:      0     461     474 
   Size:        461      13
 
HpaI GTT'AAC

Cuts at:      0     461     474 
   Size:        461      13
 
MspA1I CmG'CkG

Cuts at:      0      33     258     474 
   Size:         33     225     216

  Fragments arranged by size: 

                 225     216      33
 
NspI r_CATG'y

Cuts at:      0     154     474 
   Size:        154     320
 
PciI A'CATG_T

Cuts at:      0     150     474 
   Size:        150     324
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     429     474 
   Size:        429      45
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     172     204     474 
   Size:        172      32     270

  Fragments arranged by size: 

                 270     172      32
 
PstI C_TGCA'G

Cuts at:      0     257     474 
   Size:        257     217
 
PvuII CAG'CTG

Cuts at:      0     258     474 
   Size:        258     216
 
SfcI C'TryA_G

Cuts at:      0     253     474 
   Size:        253     221
 
SmlI C'TyrA_G

Cuts at:      0     163     474 
   Size:        163     311
 
TatI w'GTAC_w

Cuts at:      0     284     474 
   Size:        284     190
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     331     474 
   Size:        331     143
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AvaI    BbvCI     BglI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI     BsmI    BsrGI    BstXI    BtgZI
     BtsI     EarI  Eco57MI    EcoRV    Hin4I   HincII     HpaI   MspA1I
     NspI     PciI    PflMI     PsrI     PstI    PvuII     SfcI     SmlI
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII  HindIII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI