(Linear) (Six-base) MAPSORT of: KIEE1640.seq  Check: 1429  from: 1  to: 465



KIEE1640.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

ApoI r'AATT_y

Cuts at:      0     102     465 
   Size:        102     363
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     403     436     465 
   Size:        403      33      29

  Fragments arranged by size: 

                 403      33      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      19     465 
   Size:         19     446
 
BglI GCCn_nnn'nGGC

Cuts at:      0     290     465 
   Size:        290     175
 
BlpI GC'TnA_GC

Cuts at:      0      43     465 
   Size:         43     422
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     424     465 
   Size:         70     354      41

  Fragments arranged by size: 

                 354      70      41
 
BsaWI w'CCGG_w

Cuts at:      0     158     465 
   Size:        158     307
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     262     292     465 
   Size:        262      30     173

  Fragments arranged by size: 

                 262     173      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     414     465 
   Size:        414      51
 
BsrDI GCAATG_nn'

Cuts at:      0     240     465 
   Size:        240     225
 
BstYI r'GATC_y

Cuts at:      0     353     465 
   Size:        353     112
 
BtgI C'CryG_G

Cuts at:      0     422     465 
   Size:        422      43
 
BtsI GCAGTG_nn'

Cuts at:      0      28     381     465 
   Size:         28     353      84

  Fragments arranged by size: 

                 353      84      28
 
DraIII CAC_nnn'GTG

Cuts at:      0     459     465 
   Size:        459       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     424     465 
   Size:         70     354      41

  Fragments arranged by size: 

                 354      70      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     185     240     465 
   Size:        185      55     225

  Fragments arranged by size: 

                 225     185      55
 
EcoRV GAT'ATC

Cuts at:      0     253     465 
   Size:        253     212
 
FspI TGC'GCA

Cuts at:      0     367     465 
   Size:        367      98
 
HincII GTy'rAC

Cuts at:      0     141     465 
   Size:        141     324
 
MslI CAynn'nnrTG

Cuts at:      0     233     465 
   Size:        233     232
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     459     465 
   Size:        459       6
 
PfoI T'CCnGG_A

Cuts at:      0      90     465 
   Size:         90     375
 
PpuMI rG'GwC_Cy

Cuts at:      0     185     240     465 
   Size:        185      55     225

  Fragments arranged by size: 

                 225     185      55
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     304     336     465 
   Size:        304      32     129

  Fragments arranged by size: 

                 304     129      32
 
StyI C'CwwG_G

Cuts at:      0     217     465 
   Size:        217     248
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     262     294     465 
   Size:        262      32     171

  Fragments arranged by size: 

                 262     171      32
 

 Enzymes that do cut:

     ApoI     BaeI     BbsI     BglI     BlpI     BpmI    BsaWI    BsaXI
     BsgI    BsrDI    BstYI     BtgI     BtsI   DraIII  Eco57MI EcoO109I
    EcoRV     FspI   HincII     MslI    PflMI     PfoI    PpuMI     PsrI
     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI