(Linear) (Six-base) MAPSORT of: KIEE1639.seq  Check: 316  from: 1  to: 456



KIEE1639.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AccI GT'mk_AC

Cuts at:      0     197     456 
   Size:        197     259
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     108     456 
   Size:        108     348
 
AflIII A'CryG_T

Cuts at:      0     200     456 
   Size:        200     256
 
BclI T'GATC_A

Cuts at:      0     287     456 
   Size:        287     169
 
BglI GCCn_nnn'nGGC

Cuts at:      0     374     456 
   Size:        374      82
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     358     456 
   Size:        358      98
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      36     456 
   Size:         36     420
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     456 
   Size:        264     192
 
BsaAI yAC'GTr

Cuts at:      0     365     456 
   Size:        365      91
 
BsaBI GATnn'nnATC

Cuts at:      0     115     456 
   Size:        115     341
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     138     456 
   Size:        138     318
 
BseYI C'CCAG_C

Cuts at:      0      35     456 
   Size:         35     421
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     196     456 
   Size:         30     166     260

  Fragments arranged by size: 

                 260     166      30
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     218     456 
   Size:        218     238
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     456 
   Size:         19     437
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     108     264     456 
   Size:        108     156     192

  Fragments arranged by size: 

                 192     156     108
 
HaeII r_GCGC'y

Cuts at:      0      18     456 
   Size:         18     438
 
NspI r_CATG'y

Cuts at:      0     204     456 
   Size:        204     252
 
PciI A'CATG_T

Cuts at:      0     200     456 
   Size:        200     256
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     233     456 
   Size:        233     223
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     BclI     BglI     BmrI     BplI     BpmI
    BsaAI    BsaBI    BseRI    BseYI     BsgI    BspMI     EciI  Eco57MI
    HaeII     NspI     PciI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI