(Linear) (Six-base) MAPSORT of: KIEE1638.seq  Check: 21  from: 1  to: 456



KIEE1638.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AflIII A'CryG_T

Cuts at:      0     188     456 
   Size:        188     268
 
AleI CACnn'nnGTG

Cuts at:      0     385     456 
   Size:        385      71
 
BanII G_rGCy'C

Cuts at:      0     180     348     456 
   Size:        180     168     108

  Fragments arranged by size: 

                 180     168     108
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     128     212     456 
   Size:        128      84     244

  Fragments arranged by size: 

                 244     128      84
 
Bme1580I G_kGCm'C

Cuts at:      0     155     382     456 
   Size:        155     227      74

  Fragments arranged by size: 

                 227     155      74
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     356     456 
   Size:        356     100
 
BseYI C'CCAG_C

Cuts at:      0     325     456 
   Size:        325     131
 
BsiHKAI G_wGCw'C

Cuts at:      0     394     456 
   Size:        394      62
 
BsmI GAATG_Cn'

Cuts at:      0     167     456 
   Size:        167     289
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     180     348     382     394     456 
   Size:        155      25     168      34      12      62

  Fragments arranged by size: 

                 168     155      62      34      25      12
 
BsrGI T'GTAC_A

Cuts at:      0     128     456 
   Size:        128     328
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     409     456 
   Size:        409      47
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     342     456 
   Size:        342     114
 
BtsI GCAGTG_nn'

Cuts at:      0     441     456 
   Size:        441      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      13     456 
   Size:         13     443
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     210     264     456 
   Size:        173      37      54     192

  Fragments arranged by size: 

                 192     173      54      37
 
MslI CAynn'nnrTG

Cuts at:      0     385     456 
   Size:        385      71
 
PasI CC'CwG_GG

Cuts at:      0     348     456 
   Size:        348     108
 
PsiI TTA'TAA

Cuts at:      0      58     456 
   Size:         58     398
 
StyI C'CwwG_G

Cuts at:      0      89     456 
   Size:         89     367
 
TatI w'GTAC_w

Cuts at:      0     104     128     456 
   Size:        104      24     328

  Fragments arranged by size: 

                 328     104      24
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      57     363     456 
   Size:         57     306      93

  Fragments arranged by size: 

                 306      93      57
 

 Enzymes that do cut:

   AflIII     AleI    BanII    BciVI Bme1580I    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BsrGI    BstXI    BtgZI     BtsI    EcoNI     MmeI
     MslI     PasI     PsiI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI