(Linear) (Six-base) MAPSORT of: KIEE1634.seq  Check: 5890  from: 1  to: 447



KIEE1634.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:58  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     262     447 
   Size:        262     185
 
ApaI G_GGCC'C

Cuts at:      0      54     447 
   Size:         54     393
 
AscI GG'CGCG_CC

Cuts at:      0     132     447 
   Size:        132     315
 
BamHI G'GATC_C

Cuts at:      0     149     447 
   Size:        149     298
 
BanII G_rGCy'C

Cuts at:      0      54     447 
   Size:         54     393
 
Bme1580I G_kGCm'C

Cuts at:      0      54     447 
   Size:         54     393
 
BsaWI w'CCGG_w

Cuts at:      0     146     447 
   Size:        146     301
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     447 
   Size:        268     179
 
BsiHKAI G_wGCw'C

Cuts at:      0     128     447 
   Size:        128     319
 
Bsp1286I G_dGCh'C

Cuts at:      0      54     128     447 
   Size:         54      74     319

  Fragments arranged by size: 

                 319      74      54
 
BsrBI CCG'CTC

Cuts at:      0     164     447 
   Size:        164     283
 
BsrFI r'CCGG_y

Cuts at:      0      31     447 
   Size:         31     416
 
BssHII G'CGCG_C

Cuts at:      0     132     447 
   Size:        132     315
 
BstYI r'GATC_y

Cuts at:      0     149     447 
   Size:        149     298
 
EaeI y'GGCC_r

Cuts at:      0      29     447 
   Size:         29     418
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51     325     447 
   Size:         51     274     122

  Fragments arranged by size: 

                 274     122      51
 
EcoRV GAT'ATC

Cuts at:      0     428     447 
   Size:        428      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     159     191     447 
   Size:        159      32     256

  Fragments arranged by size: 

                 256     159      32
 
MspA1I CmG'CkG

Cuts at:      0      58     447 
   Size:         58     389
 
NaeI GCC'GGC

Cuts at:      0      33     447 
   Size:         33     414
 
NgoMIV G'CCGG_C

Cuts at:      0      31     447 
   Size:         31     416
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     116     447 
   Size:        116     331
 
PfoI T'CCnGG_A

Cuts at:      0     152     266     447 
   Size:        152     114     181

  Fragments arranged by size: 

                 181     152     114
 
PspOMI G'GGCC_C

Cuts at:      0      50     447 
   Size:         50     397
 
StyI C'CwwG_G

Cuts at:      0      69     321     447 
   Size:         69     252     126

  Fragments arranged by size: 

                 252     126      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      82     104     447 
   Size:         82      22     343

  Fragments arranged by size: 

                 343      82      22
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     207     239     447 
   Size:        207      32     208

  Fragments arranged by size: 

                 208     207      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     109     264     447 
   Size:        109     155     183

  Fragments arranged by size: 

                 183     155     109
 

 Enzymes that do cut:

     AlfI     ApaI     AscI    BamHI    BanII Bme1580I    BsaWI     BsgI
  BsiHKAI Bsp1286I    BsrBI    BsrFI   BssHII    BstYI     EaeI EcoO109I
    EcoRV    Hin4I   MspA1I     NaeI   NgoMIV    PflMI     PfoI   PspOMI
     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI