(Linear) (Six-base) MAPSORT of: KIEE1631.seq  Check: 9918  from: 1  to: 441



KIEE1631.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AclI AA'CG_TT

Cuts at:      0     377     441 
   Size:        377      64
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     131     441 
   Size:        131     310
 
AlwNI CAG_nnn'CTG

Cuts at:      0     146     441 
   Size:        146     295
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      24     284     441 
   Size:         24     260     157

  Fragments arranged by size: 

                 260     157      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     441 
   Size:         66     375
 
Bpu10I CC'TnA_GC

Cuts at:      0      71     441 
   Size:         71     370
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     177     441 
   Size:        177     264
 
BseYI C'CCAG_C

Cuts at:      0      39     104     441 
   Size:         39      65     337

  Fragments arranged by size: 

                 337      65      39
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     159     441 
   Size:        159     282
 
BspHI T'CATG_A

Cuts at:      0     158     441 
   Size:        158     283
 
BstEII G'GTnAC_C

Cuts at:      0     303     441 
   Size:        303     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     441 
   Size:         66     375
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      70     441 
   Size:         70     371
 
EcoO109I rG'GnC_Cy

Cuts at:      0     171     329     441 
   Size:        171     158     112

  Fragments arranged by size: 

                 171     158     112
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     308     441 
   Size:        308     133
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      54      86     441 
   Size:         54      32     355

  Fragments arranged by size: 

                 355      54      32
 
MslI CAynn'nnrTG

Cuts at:      0     113     441 
   Size:        113     328
 
MspA1I CmG'CkG

Cuts at:      0     364     441 
   Size:        364      77
 
PpuMI rG'GwC_Cy

Cuts at:      0     329     441 
   Size:        329     112
 
SanDI GG'GwC_CC

Cuts at:      0     329     441 
   Size:        329     112
 

 Enzymes that do cut:

     AclI     AlfI    AlwNI     BbsI     BpmI   Bpu10I    BseRI    BseYI
    BsmBI    BspHI   BstEII  Eco57MI    EcoNI EcoO109I     FalI    Hin4I
     MslI   MspA1I    PpuMI    SanDI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI