(Linear) (Six-base) MAPSORT of: KIEE1630.seq  Check: 8348  from: 1  to: 441



KIEE1630.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     441 
   Size:         28     413
 
AlwNI CAG_nnn'CTG

Cuts at:      0      73     441 
   Size:         73     368
 
ApoI r'AATT_y

Cuts at:      0     215     441 
   Size:        215     226
 
AvaI C'yCGr_G

Cuts at:      0     413     441 
   Size:        413      28
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     266     441 
   Size:         35     231     175

  Fragments arranged by size: 

                 231     175      35
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     284     441 
   Size:        284     157
 
BsaBI GATnn'nnATC

Cuts at:      0     338     441 
   Size:        338     103
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      58     441 
   Size:         58     383
 
BstYI r'GATC_y

Cuts at:      0     374     441 
   Size:        374      67
 
Bsu36I CC'TnA_GG

Cuts at:      0     194     441 
   Size:        194     247
 
EarI CTCTTCn'nnn_

Cuts at:      0       4     441 
   Size:          4     437
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28      35     266     441 
   Size:         28       7     231     175

  Fragments arranged by size: 

                 231     175      28       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45      70     441 
   Size:         45      25     371

  Fragments arranged by size: 

                 371      45      25
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     441 
   Size:         57     384
 
PpuMI rG'GwC_Cy

Cuts at:      0      45      70     441 
   Size:         45      25     371

  Fragments arranged by size: 

                 371      45      25
 
SanDI GG'GwC_CC

Cuts at:      0      45     441 
   Size:         45     396
 
ScaI AGT'ACT

Cuts at:      0     305     441 
   Size:        305     136
 
SspI AAT'ATT

Cuts at:      0     231     441 
   Size:        231     210
 
TatI w'GTAC_w

Cuts at:      0     303     398     441 
   Size:        303      95      43

  Fragments arranged by size: 

                 303      95      43
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI     BpmI     BsaI    BsaBI    BspMI
    BstYI   Bsu36I     EarI  Eco57MI EcoO109I     MmeI    PpuMI    SanDI
     ScaI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI