Restriction map for
pFN21AE1629
(Linear) (Six-base) MAPSORT of: KIEE1629.seq Check: 8135 from: 1 to: 438
KIEE1629.orf
With 182 enzymes: SgfI *
July 15, 2010 18:43 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 438
Size: 130 308
AlwNI CAG_nnn'CTG
Cuts at: 0 96 339 438
Size: 96 243 99
Fragments arranged by size:
243 99 96
BanI G'GyrC_C
Cuts at: 0 76 438
Size: 76 362
BanII G_rGCy'C
Cuts at: 0 308 438
Size: 308 130
BmgBI CAC'GTC
Cuts at: 0 381 438
Size: 381 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 27 438
Size: 27 411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 438
Size: 259 179
Bpu10I CC'TnA_GC
Cuts at: 0 145 307 438
Size: 145 162 131
Fragments arranged by size:
162 145 131
BsaHI Gr'CG_yC
Cuts at: 0 359 438
Size: 359 79
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 438
Size: 19 30 389
Fragments arranged by size:
389 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 40 364 424 438
Size: 37 3 324 60 14
Fragments arranged by size:
324 60 37 14 3
BseYI C'CCAG_C
Cuts at: 0 96 140 327 438
Size: 96 44 187 111
Fragments arranged by size:
187 111 96 44
BsiHKAI G_wGCw'C
Cuts at: 0 308 438
Size: 308 130
Bsp1286I G_dGCh'C
Cuts at: 0 308 438
Size: 308 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 240 254 438
Size: 240 14 184
Fragments arranged by size:
240 184 14
BsrFI r'CCGG_y
Cuts at: 0 4 232 438
Size: 4 228 206
Fragments arranged by size:
228 206 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 339 438
Size: 339 99
Bsu36I CC'TnA_GG
Cuts at: 0 20 438
Size: 20 418
BtgI C'CryG_G
Cuts at: 0 53 116 296 317 438
Size: 53 63 180 21 121
Fragments arranged by size:
180 121 63 53 21
DrdI GACnn_nn'nnGTC
Cuts at: 0 250 438
Size: 250 188
EaeI y'GGCC_r
Cuts at: 0 2 285 438
Size: 2 283 153
Fragments arranged by size:
283 153 2
EcoICRI GAG'CTC
Cuts at: 0 306 438
Size: 306 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 259 438
Size: 130 129 179
Fragments arranged by size:
179 130 129
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 438
Size: 133 305
EcoO109I rG'GnC_Cy
Cuts at: 0 243 438
Size: 243 195
FseI GG_CCGG'CC
Cuts at: 0 236 438
Size: 236 202
HaeII r_GCGC'y
Cuts at: 0 109 438
Size: 109 329
HincII GTy'rAC
Cuts at: 0 60 168 438
Size: 60 108 270
Fragments arranged by size:
270 108 60
MslI CAynn'nnrTG
Cuts at: 0 295 438
Size: 295 143
MspA1I CmG'CkG
Cuts at: 0 55 339 438
Size: 55 284 99
Fragments arranged by size:
284 99 55
NaeI GCC'GGC
Cuts at: 0 234 438
Size: 234 204
NcoI C'CATG_G
Cuts at: 0 317 438
Size: 317 121
NgoMIV G'CCGG_C
Cuts at: 0 232 438
Size: 232 206
NruI TCG'CGA
Cuts at: 0 418 438
Size: 418 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 438
Size: 96 342
PfoI T'CCnGG_A
Cuts at: 0 197 438
Size: 197 241
PpuMI rG'GwC_Cy
Cuts at: 0 243 438
Size: 243 195
PstI C_TGCA'G
Cuts at: 0 251 438
Size: 251 187
SacI G_AGCT'C
Cuts at: 0 308 438
Size: 308 130
SacII CC_GC'GG
Cuts at: 0 56 438
Size: 56 382
SbfI CC_TGCA'GG
Cuts at: 0 251 438
Size: 251 187
SfcI C'TryA_G
Cuts at: 0 247 438
Size: 247 191
StyI C'CwwG_G
Cuts at: 0 317 438
Size: 317 121
TatI w'GTAC_w
Cuts at: 0 35 438
Size: 35 403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 234 324 438
Size: 234 90 114
Fragments arranged by size:
234 114 90
Enzymes that do cut:
AcuI AlwNI BanI BanII BmgBI BmrI BpmI Bpu10I
BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrFI
BstAPI Bsu36I BtgI DrdI EaeI EcoICRI Eco57MI EcoNI
EcoO109I FseI HaeII HincII MslI MspA1I NaeI NcoI
NgoMIV NruI PflMI PfoI PpuMI PstI SacI SacII
SbfI SfcI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
EagI EarI EciI EcoRI EcoRV FalI FspI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NdeI NheI NotI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI XmnI ZraI