(Linear) (Six-base) MAPSORT of: KIEE1628.seq  Check: 8937  from: 1  to: 432



KIEE1628.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     432 
   Size:        400      32
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     116     432 
   Size:        116     316
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15     246     432 
   Size:         15     231     186

  Fragments arranged by size: 

                 231     186      15
 
ApaI G_GGCC'C

Cuts at:      0     277     432 
   Size:        277     155
 
BanII G_rGCy'C

Cuts at:      0     277     432 
   Size:        277     155
 
BglI GCCn_nnn'nGGC

Cuts at:      0     251     432 
   Size:        251     181
 
Bme1580I G_kGCm'C

Cuts at:      0      31     277     432 
   Size:         31     246     155

  Fragments arranged by size: 

                 246     155      31
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     247     432 
   Size:        181      66     185

  Fragments arranged by size: 

                 185     181      66
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     432 
   Size:        253     179
 
BsaHI Gr'CG_yC

Cuts at:      0     120     432 
   Size:        120     312
 
BseYI C'CCAG_C

Cuts at:      0      67      73     413     432 
   Size:         67       6     340      19

  Fragments arranged by size: 

                 340      67      19       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     432 
   Size:         35     397
 
BsmI GAATG_Cn'

Cuts at:      0     105     432 
   Size:        105     327
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     127     432 
   Size:        127     305
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     277     432 
   Size:         31     246     155

  Fragments arranged by size: 

                 246     155      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     217     432 
   Size:        217     215
 
BsrBI CCG'CTC

Cuts at:      0      62     432 
   Size:         62     370
 
BsrGI T'GTAC_A

Cuts at:      0     233     432 
   Size:        233     199
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      15     246     432 
   Size:         15     231     186

  Fragments arranged by size: 

                 231     186      15
 
BtsI GCAGTG_nn'

Cuts at:      0     143     309     432 
   Size:        143     166     123

  Fragments arranged by size: 

                 166     143     123
 
EaeI y'GGCC_r

Cuts at:      0     290     432 
   Size:        290     142
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     247     400     432 
   Size:        181      66     153      32

  Fragments arranged by size: 

                 181     153      66      32
 
EcoO109I rG'GnC_Cy

Cuts at:      0     243     273     274     432 
   Size:        243      30       1     158

  Fragments arranged by size: 

                 243     158      30       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     294     432 
   Size:        294     138
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     432 
   Size:        343      89
 
MscI TGG'CCA

Cuts at:      0     292     432 
   Size:        292     140
 
MslI CAynn'nnrTG

Cuts at:      0      98     432 
   Size:         98     334
 
NspI r_CATG'y

Cuts at:      0      97     432 
   Size:         97     335
 
PasI CC'CwG_GG

Cuts at:      0      86     432 
   Size:         86     346
 
PfoI T'CCnGG_A

Cuts at:      0     107     197     425     432 
   Size:        107      90     228       7

  Fragments arranged by size: 

                 228     107      90       7
 
PspOMI G'GGCC_C

Cuts at:      0     273     432 
   Size:        273     159
 
PstI C_TGCA'G

Cuts at:      0      11      43     432 
   Size:         11      32     389

  Fragments arranged by size: 

                 389      32      11
 
SfcI C'TryA_G

Cuts at:      0       7      39     214     432 
   Size:          7      32     175     218

  Fragments arranged by size: 

                 218     175      32       7
 
SmlI C'TyrA_G

Cuts at:      0     268     432 
   Size:        268     164
 
TatI w'GTAC_w

Cuts at:      0     233     432 
   Size:        233     199
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI    BanII     BglI Bme1580I     BpmI
    BpuEI    BsaHI    BseYI     BsgI     BsmI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrGI   BstAPI     BtsI     EaeI  Eco57MI EcoO109I     FalI
     MmeI     MscI     MslI     NspI     PasI     PfoI   PspOMI     PstI
     SfcI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BspEI    BspHI    BsrDI
    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI