(Linear) (Six-base) MAPSORT of: KIEE1626.seq  Check: 7389  from: 1  to: 426



KIEE1626.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AleI CACnn'nnGTG

Cuts at:      0     155     426 
   Size:        155     271
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     139     426 
   Size:        139     287
 
AlwNI CAG_nnn'CTG

Cuts at:      0      90     426 
   Size:         90     336
 
BglI GCCn_nnn'nGGC

Cuts at:      0      52     426 
   Size:         52     374
 
BseYI C'CCAG_C

Cuts at:      0      83     426 
   Size:         83     343
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     426 
   Size:        372      54
 
BstYI r'GATC_y

Cuts at:      0     338     426 
   Size:        338      88
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     102     426 
   Size:        102     324
 
EaeI y'GGCC_r

Cuts at:      0      53     426 
   Size:         53     373
 
EarI CTCTTCn'nnn_

Cuts at:      0     217     426 
   Size:        217     209
 
EcoO109I rG'GnC_Cy

Cuts at:      0     252     426 
   Size:        252     174
 
HindIII A'AGCT_T

Cuts at:      0     229     426 
   Size:        229     197
 
MscI TGG'CCA

Cuts at:      0      55     426 
   Size:         55     371
 
MslI CAynn'nnrTG

Cuts at:      0     155     426 
   Size:        155     271
 
MspA1I CmG'CkG

Cuts at:      0      87     426 
   Size:         87     339
 
PfoI T'CCnGG_A

Cuts at:      0     296     341     426 
   Size:        296      45      85

  Fragments arranged by size: 

                 296      85      45
 
PpuMI rG'GwC_Cy

Cuts at:      0     252     426 
   Size:        252     174
 
PvuII CAG'CTG

Cuts at:      0      87     426 
   Size:         87     339
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     258     290     426 
   Size:        258      32     136

  Fragments arranged by size: 

                 258     136      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     339     426 
   Size:        339      87
 

 Enzymes that do cut:

     AleI     AlfI    AlwNI     BglI    BseYI     BsgI    BstYI    BtgZI
     EaeI     EarI EcoO109I  HindIII     MscI     MslI   MspA1I     PfoI
    PpuMI    PvuII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI