(Linear) (Six-base) MAPSORT of: KIEE1625.seq  Check: 5872  from: 1  to: 426



KIEE1625.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AatII G_ACGT'C

Cuts at:      0     167     426 
   Size:        167     259
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406     426 
   Size:        406      20
 
ApaLI G'TGCA_C

Cuts at:      0     153     426 
   Size:        153     273
 
ApoI r'AATT_y

Cuts at:      0     318     426 
   Size:        318     108
 
BclI T'GATC_A

Cuts at:      0     338     426 
   Size:        338      88
 
Bme1580I G_kGCm'C

Cuts at:      0     157     426 
   Size:        157     269
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     426 
   Size:        379      47
 
Bpu10I CC'TnA_GC

Cuts at:      0     326     426 
   Size:        326     100
 
BsaAI yAC'GTr

Cuts at:      0     239     426 
   Size:        239     187
 
BsaHI Gr'CG_yC

Cuts at:      0     164     413     426 
   Size:        164     249      13

  Fragments arranged by size: 

                 249     164      13
 
BsaWI w'CCGG_w

Cuts at:      0     167     426 
   Size:        167     259
 
BsiEI CG_ry'CG

Cuts at:      0      68     426 
   Size:         68     358
 
BsiHKAI G_wGCw'C

Cuts at:      0     157     426 
   Size:        157     269
 
Bsp1286I G_dGCh'C

Cuts at:      0     157     426 
   Size:        157     269
 
BsrBI CCG'CTC

Cuts at:      0     127     426 
   Size:        127     299
 
BssHII G'CGCG_C

Cuts at:      0     270     426 
   Size:        270     156
 
BtsI GCAGTG_nn'

Cuts at:      0     157     426 
   Size:        157     269
 
DraI TTT'AAA

Cuts at:      0      87     426 
   Size:         87     339
 
EaeI y'GGCC_r

Cuts at:      0       2      65     426 
   Size:          2      63     361

  Fragments arranged by size: 

                 361      63       2
 
EagI C'GGCC_G

Cuts at:      0      65     426 
   Size:         65     361
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     406     426 
   Size:        379      27      20

  Fragments arranged by size: 

                 379      27      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     301     426 
   Size:        301     125
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     330     362     426 
   Size:         46      32     252      32      64

  Fragments arranged by size: 

                 252      64      46      32      32
 
MscI TGG'CCA

Cuts at:      0       4     426 
   Size:          4     422
 
MslI CAynn'nnrTG

Cuts at:      0      76     426 
   Size:         76     350
 
MspA1I CmG'CkG

Cuts at:      0     260     426 
   Size:        260     166
 
PmlI CAC'GTG

Cuts at:      0     239     426 
   Size:        239     187
 
PstI C_TGCA'G

Cuts at:      0     269     426 
   Size:        269     157
 
SexAI A'CCwGG_T

Cuts at:      0      44     426 
   Size:         44     382
 
SfcI C'TryA_G

Cuts at:      0     265     426 
   Size:        265     161
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      66     426 
   Size:         66     360
 
ZraI GAC'GTC

Cuts at:      0     165     426 
   Size:        165     261
 

 Enzymes that do cut:

    AatII     AcuI    ApaLI     ApoI     BclI Bme1580I     BpmI   Bpu10I
    BsaAI    BsaHI    BsaWI    BsiEI  BsiHKAI Bsp1286I    BsrBI   BssHII
     BtsI     DraI     EaeI     EagI  Eco57MI    EcoNI    Hin4I     MscI
     MslI   MspA1I     PmlI     PstI    SexAI     SfcI    TaqII     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaXI
    BseRI    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI