(Linear) (Six-base) MAPSORT of: KIEE1621.seq  Check: 5548  from: 1  to: 405



KIEE1621.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

ApoI r'AATT_y

Cuts at:      0     123     310     405 
   Size:        123     187      95

  Fragments arranged by size: 

                 187     123      95
 
BanII G_rGCy'C

Cuts at:      0     110     264     405 
   Size:        110     154     141

  Fragments arranged by size: 

                 154     141     110
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     362     405 
   Size:        362      43
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     274     405 
   Size:         55     219     131

  Fragments arranged by size: 

                 219     131      55
 
BsaWI w'CCGG_w

Cuts at:      0     351     405 
   Size:        351      54
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      40     405 
   Size:         40     365
 
BseYI C'CCAG_C

Cuts at:      0      91     230     405 
   Size:         91     139     175

  Fragments arranged by size: 

                 175     139      91
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     405 
   Size:        180     225
 
BsiHKAI G_wGCw'C

Cuts at:      0      32     405 
   Size:         32     373
 
Bsp1286I G_dGCh'C

Cuts at:      0      32     110     264     405 
   Size:         32      78     154     141

  Fragments arranged by size: 

                 154     141      78      32
 
BspEI T'CCGG_A

Cuts at:      0     351     405 
   Size:        351      54
 
BspHI T'CATG_A

Cuts at:      0     174     405 
   Size:        174     231
 
DraI TTT'AAA

Cuts at:      0     315     405 
   Size:        315      90
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     405 
   Size:         19     386
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     274     405 
   Size:         55     219     131

  Fragments arranged by size: 

                 219     131      55
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     249     405 
   Size:        249     156
 

 Enzymes that do cut:

     ApoI    BanII     BbsI     BpmI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspEI    BspHI     DraI     EciI  Eco57MI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI