Restriction map for
pFN21AE1619
(Linear) (Six-base) MAPSORT of: KIEE1619.seq Check: 8771 from: 1 to: 396
KIEE1619.orf
With 182 enzymes: SgfI *
July 15, 2010 18:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 282 396
Size: 282 114
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 396
Size: 172 224
AlwNI CAG_nnn'CTG
Cuts at: 0 111 396
Size: 111 285
ApaI G_GGCC'C
Cuts at: 0 147 396
Size: 147 249
BanI G'GyrC_C
Cuts at: 0 294 396
Size: 294 102
BanII G_rGCy'C
Cuts at: 0 63 125 147 242 396
Size: 63 62 22 95 154
Fragments arranged by size:
154 95 63 62 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 163 396
Size: 163 233
BlpI GC'TnA_GC
Cuts at: 0 19 396
Size: 19 377
Bme1580I G_kGCm'C
Cuts at: 0 30 147 396
Size: 30 117 249
Fragments arranged by size:
249 117 30
BmgBI CAC'GTC
Cuts at: 0 253 396
Size: 253 143
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 261 396
Size: 261 135
Bpu10I CC'TnA_GC
Cuts at: 0 200 396
Size: 200 196
BsaAI yAC'GTr
Cuts at: 0 320 396
Size: 320 76
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 71 237 396
Size: 71 166 159
Fragments arranged by size:
166 159 71
BseYI C'CCAG_C
Cuts at: 0 363 396
Size: 363 33
BsiHKAI G_wGCw'C
Cuts at: 0 125 396
Size: 125 271
Bsp1286I G_dGCh'C
Cuts at: 0 30 63 125 147 242 396
Size: 30 33 62 22 95 154
Fragments arranged by size:
154 95 62 33 30 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 233 282 396
Size: 233 49 114
Fragments arranged by size:
233 114 49
BsrBI CCG'CTC
Cuts at: 0 245 396
Size: 245 151
BstAPI GCAn_nnn'nTGC
Cuts at: 0 46 396
Size: 46 350
BstYI r'GATC_y
Cuts at: 0 183 396
Size: 183 213
BtgI C'CryG_G
Cuts at: 0 80 396
Size: 80 316
BtsI GCAGTG_nn'
Cuts at: 0 40 396
Size: 40 356
EarI CTCTTCn'nnn_
Cuts at: 0 173 396
Size: 173 223
EcoICRI GAG'CTC
Cuts at: 0 123 396
Size: 123 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 396
Size: 172 224
EcoO109I rG'GnC_Cy
Cuts at: 0 108 144 396
Size: 108 36 252
Fragments arranged by size:
252 108 36
MspA1I CmG'CkG
Cuts at: 0 13 396
Size: 13 383
NcoI C'CATG_G
Cuts at: 0 80 396
Size: 80 316
PmlI CAC'GTG
Cuts at: 0 320 396
Size: 320 76
PspOMI G'GGCC_C
Cuts at: 0 143 396
Size: 143 253
PstI C_TGCA'G
Cuts at: 0 168 230 388 396
Size: 168 62 158 8
Fragments arranged by size:
168 158 62 8
SacI G_AGCT'C
Cuts at: 0 125 396
Size: 125 271
SbfI CC_TGCA'GG
Cuts at: 0 230 396
Size: 230 166
SfcI C'TryA_G
Cuts at: 0 164 226 384 396
Size: 164 62 158 12
Fragments arranged by size:
164 158 62 12
StuI AGG'CCT
Cuts at: 0 99 131 205 396
Size: 99 32 74 191
Fragments arranged by size:
191 99 74 32
StyI C'CwwG_G
Cuts at: 0 80 206 396
Size: 80 126 190
Fragments arranged by size:
190 126 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 278 310 396
Size: 278 32 86
Fragments arranged by size:
278 86 32
Enzymes that do cut:
AarI AcuI AlwNI ApaI BanI BanII BbsI BlpI
Bme1580I BmgBI BplI Bpu10I BsaAI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrBI BstAPI BstYI BtgI BtsI EarI
EcoICRI Eco57MI EcoO109I MspA1I NcoI PmlI PspOMI PstI
SacI SbfI SfcI StuI StyI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmrI BmtI BpmI
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI