(Linear) (Six-base) MAPSORT of: KIEE1618.seq  Check: 2348  from: 1  to: 384



KIEE1618.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AleI CACnn'nnGTG

Cuts at:      0     314     384 
   Size:        314      70
 
AlwNI CAG_nnn'CTG

Cuts at:      0     126     384 
   Size:        126     258
 
ApoI r'AATT_y

Cuts at:      0      86     384 
   Size:         86     298
 
BanII G_rGCy'C

Cuts at:      0      56     384 
   Size:         56     328
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      22     384 
   Size:         22     362
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     115     384 
   Size:        115     269
 
BglII A'GATC_T

Cuts at:      0       5     384 
   Size:          5     379
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      50     384 
   Size:         50     334
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     384 
   Size:        169     215
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     384 
   Size:         64     320
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     384 
   Size:        184     200
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     384 
   Size:         56     328
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     368     384 
   Size:        368      16
 
BsrFI r'CCGG_y

Cuts at:      0     136     384 
   Size:        136     248
 
BssSI C'ACGA_G

Cuts at:      0     314     384 
   Size:        314      70
 
BstYI r'GATC_y

Cuts at:      0       5     384 
   Size:          5     379
 
DraIII CAC_nnn'GTG

Cuts at:      0     207     384 
   Size:        207     177
 
EaeI y'GGCC_r

Cuts at:      0     156     384 
   Size:        156     228
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     235     384 
   Size:        235     149
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     384 
   Size:        169     215
 
HincII GTy'rAC

Cuts at:      0     324     384 
   Size:        324      60
 
MslI CAynn'nnrTG

Cuts at:      0     314     384 
   Size:        314      70
 
MspA1I CmG'CkG

Cuts at:      0     147     384 
   Size:        147     237
 
NaeI GCC'GGC

Cuts at:      0     138     384 
   Size:        138     246
 
NgoMIV G'CCGG_C

Cuts at:      0     136     384 
   Size:        136     248
 
PvuII CAG'CTG

Cuts at:      0     147     384 
   Size:        147     237
 
SexAI A'CCwGG_T

Cuts at:      0     203     384 
   Size:        203     181
 
SmlI C'TyrA_G

Cuts at:      0      43     384 
   Size:         43     341
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     201     384 
   Size:        201     183
 

 Enzymes that do cut:

     AleI    AlwNI     ApoI    BanII     BbsI    BciVI    BglII     BmrI
     BpmI    BpuEI     BsgI Bsp1286I    BspMI    BsrFI    BssSI    BstYI
   DraIII     EaeI     EciI  Eco57MI   HincII     MslI   MspA1I     NaeI
   NgoMIV    PvuII    SexAI     SmlI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EagI     EarI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI