(Linear) (Six-base) MAPSORT of: KIEE1614.seq  Check: 4221  from: 1  to: 378



KIEE1614.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:57  ..

AgeI A'CCGG_T

Cuts at:      0     107     378 
   Size:        107     271
 
AhdI GACnn_n'nnGTC

Cuts at:      0     319     378 
   Size:        319      59
 
BanI G'GyrC_C

Cuts at:      0     110     378 
   Size:        110     268
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     208     378 
   Size:        208     170
 
BfrBI ATG'CAT

Cuts at:      0     363     378 
   Size:        363      15
 
Bpu10I CC'TnA_GC

Cuts at:      0      23     378 
   Size:         23     355
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     378 
   Size:        122     256
 
BsaWI w'CCGG_w

Cuts at:      0     107     378 
   Size:        107     271
 
BsrFI r'CCGG_y

Cuts at:      0     107     378 
   Size:        107     271
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     346     378 
   Size:        346      32
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     271     378 
   Size:        271     107
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     305     337     378 
   Size:        305      32      41

  Fragments arranged by size: 

                 305      41      32
 
MslI CAynn'nnrTG

Cuts at:      0     338     378 
   Size:        338      40
 
NsiI A_TGCA'T

Cuts at:      0     365     378 
   Size:        365      13
 
SmlI C'TyrA_G

Cuts at:      0     137     378 
   Size:        137     241
 
StyI C'CwwG_G

Cuts at:      0      17     378 
   Size:         17     361
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      94     126     378 
   Size:         94      32     252

  Fragments arranged by size: 

                 252      94      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     215     378 
   Size:        215     163
 

 Enzymes that do cut:

     AgeI     AhdI     BanI     BbsI    BfrBI   Bpu10I    BpuEI    BsaWI
    BsrFI    BstXI    EcoNI    Hin4I     MslI     NsiI     SmlI     StyI
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI