(Linear) (Six-base) MAPSORT of: KIEE1611.seq  Check: 4387  from: 1  to: 345



KIEE1611.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     345 
   Size:        124     221
 
AleI CACnn'nnGTG

Cuts at:      0     221     345 
   Size:        221     124
 
AlwNI CAG_nnn'CTG

Cuts at:      0     102     345 
   Size:        102     243
 
ApaLI G'TGCA_C

Cuts at:      0     273     345 
   Size:        273      72
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      22     345 
   Size:         22     323
 
BclI T'GATC_A

Cuts at:      0     110     345 
   Size:        110     235
 
Bme1580I G_kGCm'C

Cuts at:      0     247     277     345 
   Size:        247      30      68

  Fragments arranged by size: 

                 247      68      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     250     345 
   Size:        250      95
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     345 
   Size:         52     293
 
BseYI C'CCAG_C

Cuts at:      0     156     345 
   Size:        156     189
 
BsiHKAI G_wGCw'C

Cuts at:      0     277     345 
   Size:        277      68
 
Bsp1286I G_dGCh'C

Cuts at:      0     247     277     345 
   Size:        247      30      68

  Fragments arranged by size: 

                 247      68      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     251     345 
   Size:        251      94
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      24     345 
   Size:         24     321
 
EaeI y'GGCC_r

Cuts at:      0       5     345 
   Size:          5     340
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     124     345 
   Size:         52      72     221

  Fragments arranged by size: 

                 221      72      52
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     345 
   Size:         78     267
 
MslI CAynn'nnrTG

Cuts at:      0     221     345 
   Size:        221     124
 
NspI r_CATG'y

Cuts at:      0     254     324     345 
   Size:        254      70      21

  Fragments arranged by size: 

                 254      70      21
 
ScaI AGT'ACT

Cuts at:      0     184     345 
   Size:        184     161
 
SfcI C'TryA_G

Cuts at:      0     219     264     345 
   Size:        219      45      81

  Fragments arranged by size: 

                 219      81      45
 
SphI G_CATG'C

Cuts at:      0     254     324     345 
   Size:        254      70      21

  Fragments arranged by size: 

                 254      70      21
 
SspI AAT'ATT

Cuts at:      0     234     345 
   Size:        234     111
 
TatI w'GTAC_w

Cuts at:      0     182     345 
   Size:        182     163
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     220     345 
   Size:        220     125
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI    ApaLI     BbsI     BclI Bme1580I     BmrI
     BpmI    BseYI  BsiHKAI Bsp1286I   BstAPI     DrdI     EaeI  Eco57MI
     MmeI     MslI     NspI     ScaI     SfcI     SphI     SspI     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI