(Linear) (Six-base) MAPSORT of: KIEE1610.seq  Check: 1300  from: 1  to: 345



KIEE1610.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     345 
   Size:          6     339
 
AflII C'TTAA_G

Cuts at:      0      85     345 
   Size:         85     260
 
BanII G_rGCy'C

Cuts at:      0     109     345 
   Size:        109     236
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     125     345 
   Size:        125     220
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     161     345 
   Size:         71      90     184

  Fragments arranged by size: 

                 184      90      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     345 
   Size:        345
 
BsaWI w'CCGG_w

Cuts at:      0     111     345 
   Size:        111     234
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79     345 
   Size:         79     266
 
BseYI C'CCAG_C

Cuts at:      0     120     125     345 
   Size:        120       5     220

  Fragments arranged by size: 

                 220     120       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     345 
   Size:         65     280
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      47     345 
   Size:         47     298
 
Bsp1286I G_dGCh'C

Cuts at:      0     109     345 
   Size:        109     236
 
BsrDI GCAATG_nn'

Cuts at:      0     225     345 
   Size:        225     120
 
BtsI GCAGTG_nn'

Cuts at:      0     275     345 
   Size:        275      70
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     179     214     345 
   Size:        179      35     131

  Fragments arranged by size: 

                 179     131      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6      71     161     345 
   Size:          6      65      90     184

  Fragments arranged by size: 

                 184      90      65       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     229     345 
   Size:        229     116
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     270     345 
   Size:        270      75
 
MspA1I CmG'CkG

Cuts at:      0      49     345 
   Size:         49     296
 
PvuII CAG'CTG

Cuts at:      0      49     345 
   Size:         49     296
 
SfcI C'TryA_G

Cuts at:      0     250     345 
   Size:        250      95
 
SmlI C'TyrA_G

Cuts at:      0      85     324     345 
   Size:         85     239      21

  Fragments arranged by size: 

                 239      85      21
 

 Enzymes that do cut:

     AcuI    AflII    BanII     BplI     BpmI    BpuEI    BsaWI    BseRI
    BseYI     BsgI    BsmBI Bsp1286I    BsrDI     BtsI    CspCI  Eco57MI
    EcoNI     MmeI   MspA1I    PvuII     SfcI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI