(Linear) (Six-base) MAPSORT of: KIEE1605.seq  Check: 8887  from: 1  to: 327



KIEE1605.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

ApoI r'AATT_y

Cuts at:      0     149     327 
   Size:        149     178
 
BanII G_rGCy'C

Cuts at:      0     118     327 
   Size:        118     209
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       3      37     327 
   Size:          3      34     290

  Fragments arranged by size: 

                 290      34       3
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     242     327 
   Size:        242      85
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38      91     327 
   Size:         38      53     236

  Fragments arranged by size: 

                 236      53      38
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     221     327 
   Size:        111     110     106

  Fragments arranged by size: 

                 111     110     106
 
BsiHKAI G_wGCw'C

Cuts at:      0     118     327 
   Size:        118     209
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     327 
   Size:        118     209
 
BtgI C'CryG_G

Cuts at:      0     165     327 
   Size:        165     162
 
EcoICRI GAG'CTC

Cuts at:      0     116     327 
   Size:        116     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38      91     327 
   Size:         38      53     236

  Fragments arranged by size: 

                 236      53      38
 
EcoRI G'AATT_C

Cuts at:      0     149     327 
   Size:        149     178
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     302     327 
   Size:        302      25
 
HindIII A'AGCT_T

Cuts at:      0      87     327 
   Size:         87     240
 
MfeI C'AATT_G

Cuts at:      0     218     327 
   Size:        218     109
 
SacI G_AGCT'C

Cuts at:      0     118     327 
   Size:        118     209
 
SmlI C'TyrA_G

Cuts at:      0      90     236     327 
   Size:         90     146      91

  Fragments arranged by size: 

                 146      91      90
 
StyI C'CwwG_G

Cuts at:      0      60     327 
   Size:         60     267
 
XmnI GAAnn'nnTTC

Cuts at:      0     285     327 
   Size:        285      42
 

 Enzymes that do cut:

     ApoI    BanII     BcgI    BciVI     BpmI    BpuEI  BsiHKAI Bsp1286I
     BtgI  EcoICRI  Eco57MI    EcoRI     FalI  HindIII     MfeI     SacI
     SmlI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI