(Linear) (Six-base) MAPSORT of: KIEE1603.seq  Check: 3064  from: 1  to: 312



KIEE1603.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     298     312 
   Size:        190     108      14

  Fragments arranged by size: 

                 190     108      14
 
ApaI G_GGCC'C

Cuts at:      0     297     312 
   Size:        297      15
 
BanI G'GyrC_C

Cuts at:      0     154     312 
   Size:        154     158
 
BanII G_rGCy'C

Cuts at:      0      26      73     223     297     312 
   Size:         26      47     150      74      15

  Fragments arranged by size: 

                 150      74      47      26      15
 
BglI GCCn_nnn'nGGC

Cuts at:      0     262     271     280     312 
   Size:        262       9       9      32

  Fragments arranged by size: 

                 262      32       9       9
 
BlpI GC'TnA_GC

Cuts at:      0     181     239     312 
   Size:        181      58      73

  Fragments arranged by size: 

                 181      73      58
 
Bme1580I G_kGCm'C

Cuts at:      0     297     312 
   Size:        297      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     312 
   Size:        127     185
 
BseYI C'CCAG_C

Cuts at:      0      89     243     288     312 
   Size:         89     154      45      24

  Fragments arranged by size: 

                 154      89      45      24
 
Bsp1286I G_dGCh'C

Cuts at:      0      26      73     223     297     312 
   Size:         26      47     150      74      15

  Fragments arranged by size: 

                 150      74      47      26      15
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      84     312 
   Size:         84     228
 
BtgI C'CryG_G

Cuts at:      0     257     312 
   Size:        257      55
 
EaeI y'GGCC_r

Cuts at:      0      74     140     312 
   Size:         74      66     172

  Fragments arranged by size: 

                 172      74      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     133     312 
   Size:        133     179
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     190     298     312 
   Size:        127      63     108      14

  Fragments arranged by size: 

                 127     108      63      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     214     312 
   Size:        214      98
 
EcoO109I rG'GnC_Cy

Cuts at:      0     134     247     273     282     293     294     312 
   Size:        134     113      26       9      11       1      18

  Fragments arranged by size: 

                 134     113      26      18      11       9       1
 
MscI TGG'CCA

Cuts at:      0     142     312 
   Size:        142     170
 
MslI CAynn'nnrTG

Cuts at:      0     199     312 
   Size:        199     113
 
MspA1I CmG'CkG

Cuts at:      0      52     168     312 
   Size:         52     116     144

  Fragments arranged by size: 

                 144     116      52
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     258     312 
   Size:        258      54
 
PpuMI rG'GwC_Cy

Cuts at:      0     134     247     312 
   Size:        134     113      65

  Fragments arranged by size: 

                 134     113      65
 
PspOMI G'GGCC_C

Cuts at:      0     293     312 
   Size:        293      19
 
PstI C_TGCA'G

Cuts at:      0      46     312 
   Size:         46     266
 
SanDI GG'GwC_CC

Cuts at:      0     134     247     312 
   Size:        134     113      65

  Fragments arranged by size: 

                 134     113      65
 
SfcI C'TryA_G

Cuts at:      0      42     312 
   Size:         42     270
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     262     271     280     312 
   Size:        262       9       9      32

  Fragments arranged by size: 

                 262      32       9       9
 

 Enzymes that do cut:

     AcuI     ApaI     BanI    BanII     BglI     BlpI Bme1580I     BpmI
    BseYI Bsp1286I    BstXI     BtgI     EaeI     EciI  Eco57MI    EcoNI
 EcoO109I     MscI     MslI   MspA1I    PflMI    PpuMI   PspOMI     PstI
    SanDI     SfcI     SfiI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI