(Linear) (Six-base) MAPSORT of: KIEE1601.seq  Check: 3985  from: 1  to: 309



KIEE1601.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     309 
   Size:        196     113
 
BlpI GC'TnA_GC

Cuts at:      0      47     309 
   Size:         47     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     309 
   Size:        208     101
 
Bpu10I CC'TnA_GC

Cuts at:      0      30     309 
   Size:         30     279
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     256     309 
   Size:         87     169      53

  Fragments arranged by size: 

                 169      87      53
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     154     309 
   Size:        154     155
 
BsaHI Gr'CG_yC

Cuts at:      0     108     309 
   Size:        108     201
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     309 
   Size:        172     137
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     309 
   Size:        227      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     309 
   Size:        227      82
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     258     309 
   Size:        258      51
 
BstYI r'GATC_y

Cuts at:      0     137     309 
   Size:        137     172
 
BtgI C'CryG_G

Cuts at:      0     165     251     309 
   Size:        165      86      58

  Fragments arranged by size: 

                 165      86      58
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      35     309 
   Size:         35     274
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     208     309 
   Size:        196      12     101

  Fragments arranged by size: 

                 196     101      12
 
HaeII r_GCGC'y

Cuts at:      0      47      54     279     309 
   Size:         47       7     225      30

  Fragments arranged by size: 

                 225      47      30       7
 
MslI CAynn'nnrTG

Cuts at:      0     250     256     309 
   Size:        250       6      53

  Fragments arranged by size: 

                 250      53       6
 
MspA1I CmG'CkG

Cuts at:      0     167     309 
   Size:        167     142
 
NcoI C'CATG_G

Cuts at:      0     251     309 
   Size:        251      58
 
PfoI T'CCnGG_A

Cuts at:      0     185     309 
   Size:        185     124
 
SacII CC_GC'GG

Cuts at:      0     168     309 
   Size:        168     141
 
SmlI C'TyrA_G

Cuts at:      0      66     271     309 
   Size:         66     205      38

  Fragments arranged by size: 

                 205      66      38
 
StyI C'CwwG_G

Cuts at:      0     251     309 
   Size:        251      58
 
Tth111I GACn'n_nGTC

Cuts at:      0     208     309 
   Size:        208     101
 
XmnI GAAnn'nnTTC

Cuts at:      0     181     309 
   Size:        181     128
 

 Enzymes that do cut:

     AcuI     BlpI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BseRI
  BsiHKAI Bsp1286I    BstXI    BstYI     BtgI     EciI  Eco57MI    HaeII
     MslI   MspA1I     NcoI     PfoI    SacII     SmlI     StyI  Tth111I
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI
    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI     XbaI     XcmI
     XhoI     XmaI     ZraI