Restriction map for
pFN21AE1594
(Linear) (Six-base) MAPSORT of: KIEE1594.seq Check: 8761 from: 1 to: 900
KIEE1594.orf
With 182 enzymes: SgfI *
July 15, 2010 18:43 ..
AatII G_ACGT'C
Cuts at: 0 780 900
Size: 780 120
AccI GT'mk_AC
Cuts at: 0 774 900
Size: 774 126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 425 900
Size: 425 475
AlwNI CAG_nnn'CTG
Cuts at: 0 454 471 705 900
Size: 454 17 234 195
Fragments arranged by size:
454 234 195 17
ApoI r'AATT_y
Cuts at: 0 144 492 900
Size: 144 348 408
Fragments arranged by size:
408 348 144
BanII G_rGCy'C
Cuts at: 0 504 636 900
Size: 504 132 264
Fragments arranged by size:
504 264 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 258 900
Size: 258 642
BbvCI CC'TCA_GC
Cuts at: 0 736 900
Size: 736 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 900
Size: 826 74
Bpu10I CC'TnA_GC
Cuts at: 0 164 736 900
Size: 164 572 164
Fragments arranged by size:
572 164 164
BsaHI Gr'CG_yC
Cuts at: 0 777 900
Size: 777 123
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 494 900
Size: 494 406
BseYI C'CCAG_C
Cuts at: 0 71 698 900
Size: 71 627 202
Fragments arranged by size:
627 202 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 900
Size: 145 755
BsiHKAI G_wGCw'C
Cuts at: 0 504 636 900
Size: 504 132 264
Fragments arranged by size:
504 264 132
Bsp1286I G_dGCh'C
Cuts at: 0 504 636 900
Size: 504 132 264
Fragments arranged by size:
504 264 132
BsrDI GCAATG_nn'
Cuts at: 0 407 900
Size: 407 493
BsrGI T'GTAC_A
Cuts at: 0 525 900
Size: 525 375
BstAPI GCAn_nnn'nTGC
Cuts at: 0 178 900
Size: 178 722
BstXI CCAn_nnnn'nTGG
Cuts at: 0 79 900
Size: 79 821
BstYI r'GATC_y
Cuts at: 0 451 626 900
Size: 451 175 274
Fragments arranged by size:
451 274 175
BtgI C'CryG_G
Cuts at: 0 53 362 900
Size: 53 309 538
Fragments arranged by size:
538 309 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 659 900
Size: 659 241
BtsI GCAGTG_nn'
Cuts at: 0 827 900
Size: 827 73
DraIII CAC_nnn'GTG
Cuts at: 0 514 900
Size: 514 386
EaeI y'GGCC_r
Cuts at: 0 138 900
Size: 138 762
EarI CTCTTCn'nnn_
Cuts at: 0 314 900
Size: 314 586
EcoICRI GAG'CTC
Cuts at: 0 502 634 900
Size: 502 132 266
Fragments arranged by size:
502 266 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 900
Size: 826 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 261 509 900
Size: 261 248 391
Fragments arranged by size:
391 261 248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 275 900
Size: 275 625
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 618 650 900
Size: 97 32 489 32 250
Fragments arranged by size:
489 250 97 32 32
HincII GTy'rAC
Cuts at: 0 775 900
Size: 775 125
HindIII A'AGCT_T
Cuts at: 0 844 900
Size: 844 56
MfeI C'AATT_G
Cuts at: 0 176 521 900
Size: 176 345 379
Fragments arranged by size:
379 345 176
MscI TGG'CCA
Cuts at: 0 140 900
Size: 140 760
MspA1I CmG'CkG
Cuts at: 0 547 705 900
Size: 547 158 195
Fragments arranged by size:
547 195 158
NcoI C'CATG_G
Cuts at: 0 53 362 900
Size: 53 309 538
Fragments arranged by size:
538 309 53
NspI r_CATG'y
Cuts at: 0 615 900
Size: 615 285
PflMI CCAn_nnn'nTGG
Cuts at: 0 705 900
Size: 705 195
PstI C_TGCA'G
Cuts at: 0 201 476 900
Size: 201 275 424
Fragments arranged by size:
424 275 201
PvuII CAG'CTG
Cuts at: 0 547 705 900
Size: 547 158 195
Fragments arranged by size:
547 195 158
SacI G_AGCT'C
Cuts at: 0 504 636 900
Size: 504 132 264
Fragments arranged by size:
504 264 132
SalI G'TCGA_C
Cuts at: 0 773 900
Size: 773 127
SexAI A'CCwGG_T
Cuts at: 0 510 900
Size: 510 390
SfcI C'TryA_G
Cuts at: 0 197 413 472 900
Size: 197 216 59 428
Fragments arranged by size:
428 216 197 59
StyI C'CwwG_G
Cuts at: 0 53 362 420 900
Size: 53 309 58 480
Fragments arranged by size:
480 309 58 53
TatI w'GTAC_w
Cuts at: 0 525 900
Size: 525 375
ZraI GAC'GTC
Cuts at: 0 778 900
Size: 778 122
Enzymes that do cut:
AatII AccI AlfI AlwNI ApoI BanII BbsI BbvCI
BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrDI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI FalI Hin4I
HincII HindIII MfeI MscI MspA1I NcoI NspI PflMI
PstI PvuII SacI SalI SexAI SfcI StyI TatI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EciI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI