(Linear) (Six-base) MAPSORT of: KIEE1592.seq  Check: 2124  from: 1  to: 1212



KIEE1592.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37    1212 
   Size:         37    1175
 
AflIII A'CryG_T

Cuts at:      0     484     788    1212 
   Size:        484     304     424

  Fragments arranged by size: 

                 484     424     304
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     219     586    1094    1212 
   Size:        219     367     508     118

  Fragments arranged by size: 

                 508     367     219     118
 
ApoI r'AATT_y

Cuts at:      0     209     514    1103    1212 
   Size:        209     305     589     109

  Fragments arranged by size: 

                 589     305     209     109
 
AvaI C'yCGr_G

Cuts at:      0     161    1212 
   Size:        161    1051
 
BanII G_rGCy'C

Cuts at:      0    1039    1212 
   Size:       1039     173
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     242    1059    1212 
   Size:        242     817     153

  Fragments arranged by size: 

                 817     242     153
 
BbvCI CC'TCA_GC

Cuts at:      0     545    1212 
   Size:        545     667
 
BclI T'GATC_A

Cuts at:      0     836    1212 
   Size:        836     376
 
Bme1580I G_kGCm'C

Cuts at:      0     198    1212 
   Size:        198    1014
 
BmgBI CAC'GTC

Cuts at:      0     487    1212 
   Size:        487     725
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     560     664    1212 
   Size:        560     104     548

  Fragments arranged by size: 

                 560     548     104
 
Bpu10I CC'TnA_GC

Cuts at:      0     545    1212 
   Size:        545     667
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296    1212 
   Size:        296     916
 
BsaBI GATnn'nnATC

Cuts at:      0     841    1212 
   Size:        841     371
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     533     563    1212 
   Size:        533      30     649

  Fragments arranged by size: 

                 649     533      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     329    1212 
   Size:        329     883
 
BseYI C'CCAG_C

Cuts at:      0     258     360    1212 
   Size:        258     102     852

  Fragments arranged by size: 

                 852     258     102
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370    1212 
   Size:        370     842
 
BsiHKAI G_wGCw'C

Cuts at:      0     671    1212 
   Size:        671     541
 
Bsp1286I G_dGCh'C

Cuts at:      0     198     671    1039    1212 
   Size:        198     473     368     173

  Fragments arranged by size: 

                 473     368     198     173
 
BsrDI GCAATG_nn'

Cuts at:      0     513    1212 
   Size:        513     699
 
Bsu36I CC'TnA_GG

Cuts at:      0     341     378    1212 
   Size:        341      37     834

  Fragments arranged by size: 

                 834     341      37
 
BtgI C'CryG_G

Cuts at:      0      47     855    1212 
   Size:         47     808     357

  Fragments arranged by size: 

                 808     357      47
 
BtsI GCAGTG_nn'

Cuts at:      0     353     617    1212 
   Size:        353     264     595

  Fragments arranged by size: 

                 595     353     264
 
DraI TTT'AAA

Cuts at:      0     885    1212 
   Size:        885     327
 
EaeI y'GGCC_r

Cuts at:      0       2    1212 
   Size:          2    1210
 
EarI CTCTTCn'nnn_

Cuts at:      0      19     107     926     999    1204    1212 
   Size:         19      88     819      73     205       8

  Fragments arranged by size: 

                 819     205      88      73      19       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     560     664    1212 
   Size:         37     523     104     548

  Fragments arranged by size: 

                 548     523     104      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0      12    1212 
   Size:         12    1200
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     337    1193    1212 
   Size:        337     856      19

  Fragments arranged by size: 

                 856     337      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297    1212 
   Size:        265      32     915

  Fragments arranged by size: 

                 915     265      32
 
HincII GTy'rAC

Cuts at:      0     552    1212 
   Size:        552     660
 
HindIII A'AGCT_T

Cuts at:      0     400    1212 
   Size:        400     812
 
MslI CAynn'nnrTG

Cuts at:      0     201     562     568    1212 
   Size:        201     361       6     644

  Fragments arranged by size: 

                 644     361     201       6
 
NcoI C'CATG_G

Cuts at:      0      47    1212 
   Size:         47    1165
 
NdeI CA'TA_TG

Cuts at:      0    1067    1212 
   Size:       1067     145
 
NspI r_CATG'y

Cuts at:      0     206     792    1212 
   Size:        206     586     420

  Fragments arranged by size: 

                 586     420     206
 
PciI A'CATG_T

Cuts at:      0     788    1212 
   Size:        788     424
 
PpuMI rG'GwC_Cy

Cuts at:      0      12    1212 
   Size:         12    1200
 
SapI GCTCTTCn'nnn_

Cuts at:      0     999    1212 
   Size:        999     213
 
ScaI AGT'ACT

Cuts at:      0     320    1212 
   Size:        320     892
 
SfcI C'TryA_G

Cuts at:      0     143     635     926    1212 
   Size:        143     492     291     286

  Fragments arranged by size: 

                 492     291     286     143
 
SmlI C'TyrA_G

Cuts at:      0     311    1212 
   Size:        311     901
 
SphI G_CATG'C

Cuts at:      0     206    1212 
   Size:        206    1006
 
StuI AGG'CCT

Cuts at:      0     850    1212 
   Size:        850     362
 
StyI C'CwwG_G

Cuts at:      0      47    1212 
   Size:         47    1165
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      40    1212 
   Size:         40    1172
 
TatI w'GTAC_w

Cuts at:      0     318    1212 
   Size:        318     894
 
Tth111I GACn'n_nGTC

Cuts at:      0      10    1212 
   Size:         10    1202
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     853    1212 
   Size:        853     359
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     ApoI     AvaI    BanII     BbsI    BbvCI
     BclI Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI    BsaBI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrDI   Bsu36I     BtgI
     BtsI     DraI     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I
   HincII  HindIII     MslI     NcoI     NdeI     NspI     PciI    PpuMI
     SapI     ScaI     SfcI     SmlI     SphI     StuI     StyI    TaqII
     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI     BsaI
    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI     TstI     XbaI     XhoI     XmaI     XmnI
     ZraI