(Linear) (Six-base) MAPSORT of: KIEE1588.seq  Check: 9725  from: 1  to: 306



KIEE1588.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     216     306 
   Size:        216      90
 
AvaI C'yCGr_G

Cuts at:      0     191     306 
   Size:        191     115
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     143     306 
   Size:        143     163
 
BseYI C'CCAG_C

Cuts at:      0     281     306 
   Size:        281      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     306 
   Size:        100     206
 
BsmI GAATG_Cn'

Cuts at:      0      74     306 
   Size:         74     232
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     216     306 
   Size:        216      90
 
BtgI C'CryG_G

Cuts at:      0     254     306 
   Size:        254      52
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     171     306 
   Size:        171     135
 
EarI CTCTTCn'nnn_

Cuts at:      0     107     306 
   Size:        107     199
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      14     306 
   Size:         14     292
 
FspI TGC'GCA

Cuts at:      0     224     306 
   Size:        224      82
 
NcoI C'CATG_G

Cuts at:      0     254     306 
   Size:        254      52
 
NspI r_CATG'y

Cuts at:      0     250     306 
   Size:        250      56
 
SmaI CCC'GGG

Cuts at:      0     193     306 
   Size:        193     113
 
SphI G_CATG'C

Cuts at:      0     250     306 
   Size:        250      56
 
StyI C'CwwG_G

Cuts at:      0     254     306 
   Size:        254      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     104     306 
   Size:        104     202
 
XmaI C'CCGG_G

Cuts at:      0     191     306 
   Size:        191     115
 

 Enzymes that do cut:

     AarI     AvaI     BmrI    BseYI     BsgI     BsmI    BspMI     BtgI
    BtgZI     EarI    EcoNI     FspI     NcoI     NspI     SmaI     SphI
     StyI    TaqII     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI