(Linear) (Six-base) MAPSORT of: KIEE1579.seq  Check: 4327  from: 1  to: 243



KIEE1579.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

AvaI C'yCGr_G

Cuts at:      0      19     243 
   Size:         19     224
 
BanII G_rGCy'C

Cuts at:      0      18     243 
   Size:         18     225
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     236     243 
   Size:        236       7
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     169     203     243 
   Size:        169      34      40

  Fragments arranged by size: 

                 169      40      34
 
BlpI GC'TnA_GC

Cuts at:      0     154     243 
   Size:        154      89
 
BmgBI CAC'GTC

Cuts at:      0     198     243 
   Size:        198      45
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      85     243 
   Size:         85     158
 
BsaWI w'CCGG_w

Cuts at:      0      30     233     243 
   Size:         30     203      10

  Fragments arranged by size: 

                 203      30      10
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25      55     243 
   Size:         25      30     188

  Fragments arranged by size: 

                 188      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     124     127     243 
   Size:         49      75       3     116

  Fragments arranged by size: 

                 116      75      49       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     243 
   Size:        209      34
 
BsiHKAI G_wGCw'C

Cuts at:      0      18     243 
   Size:         18     225
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     217     243 
   Size:        217      26
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     243 
   Size:         18     225
 
BspEI T'CCGG_A

Cuts at:      0      30     243 
   Size:         30     213
 
BsrGI T'GTAC_A

Cuts at:      0      41     243 
   Size:         41     202
 
EarI CTCTTCn'nnn_

Cuts at:      0      19     243 
   Size:         19     224
 
EcoICRI GAG'CTC

Cuts at:      0      16     243 
   Size:         16     227
 
FspI TGC'GCA

Cuts at:      0     124     243 
   Size:        124     119
 
HincII GTy'rAC

Cuts at:      0     144     243 
   Size:        144      99
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     243 
   Size:        200      43
 
MspA1I CmG'CkG

Cuts at:      0     162     243 
   Size:        162      81
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     100     132     243 
   Size:        100      32     111

  Fragments arranged by size: 

                 111     100      32
 
PvuII CAG'CTG

Cuts at:      0     162     243 
   Size:        162      81
 
SacI G_AGCT'C

Cuts at:      0      18     243 
   Size:         18     225
 
SmaI CCC'GGG

Cuts at:      0      21     243 
   Size:         21     222
 
TatI w'GTAC_w

Cuts at:      0      41     243 
   Size:         41     202
 
XmaI C'CCGG_G

Cuts at:      0      19     243 
   Size:         19     224
 

 Enzymes that do cut:

     AvaI    BanII     BbsI     BcgI     BlpI    BmgBI     BplI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BsrGI
     EarI  EcoICRI     FspI   HincII     MmeI   MspA1I     PpiI    PvuII
     SacI     SmaI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BseYI    BsiEI    BsiWI     BsmI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI