(Linear) (Six-base) MAPSORT of: KIEE1578.seq  Check: 8090  from: 1  to: 192



KIEE1578.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:55  ..

AflII C'TTAA_G

Cuts at:      0      94     192 
   Size:         94      98
 
BanI G'GyrC_C

Cuts at:      0     151     192 
   Size:        151      41
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     166     192 
   Size:        166      26
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       7     192 
   Size:          7     185
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      97     192 
   Size:         97      95
 
EarI CTCTTCn'nnn_

Cuts at:      0      25      37      56     192 
   Size:         25      12      19     136

  Fragments arranged by size: 

                 136      25      19      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      53     192 
   Size:         53     139
 
NdeI CA'TA_TG

Cuts at:      0     109     192 
   Size:        109      83
 
SmlI C'TyrA_G

Cuts at:      0      94     192 
   Size:         94      98
 

 Enzymes that do cut:

    AflII     BanI     BsaI    BseRI    BspMI     EarI    EcoNI     NdeI
     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI