Restriction map for
pFN21AE1573
(Linear) (Six-base) MAPSORT of: KIEE1573.seq Check: 3561 from: 1 to: 2880
KIEE1573.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2754 2880
Size: 2754 126
AccI GT'mk_AC
Cuts at: 0 83 1325 2880
Size: 83 1242 1555
Fragments arranged by size:
1555 1242 83
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1783 1981 2333 2880
Size: 1271 512 198 352 547
Fragments arranged by size:
1271 547 512 352 198
AflIII A'CryG_T
Cuts at: 0 776 1105 2508 2880
Size: 776 329 1403 372
Fragments arranged by size:
1403 776 372 329
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2651 2683 2880
Size: 2651 32 197
Fragments arranged by size:
2651 197 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2533 2770 2880
Size: 2533 237 110
Fragments arranged by size:
2533 237 110
ApoI r'AATT_y
Cuts at: 0 607 1451 1582 2195 2880
Size: 607 844 131 613 685
Fragments arranged by size:
844 685 613 607 131
AseI AT'TA_AT
Cuts at: 0 1079 2880
Size: 1079 1801
AvaI C'yCGr_G
Cuts at: 0 1376 2009 2880
Size: 1376 633 871
Fragments arranged by size:
1376 871 633
BamHI G'GATC_C
Cuts at: 0 2824 2880
Size: 2824 56
BanI G'GyrC_C
Cuts at: 0 708 2880
Size: 708 2172
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 2880
Size: 310 2570
BclI T'GATC_A
Cuts at: 0 114 137 2394 2880
Size: 114 23 2257 486
Fragments arranged by size:
2257 486 114 23
BglI GCCn_nnn'nGGC
Cuts at: 0 104 2880
Size: 104 2776
BglII A'GATC_T
Cuts at: 0 1008 2041 2728 2880
Size: 1008 1033 687 152
Fragments arranged by size:
1033 1008 687 152
BmgBI CAC'GTC
Cuts at: 0 1413 2880
Size: 1413 1467
BmrI ACTGGGnnnn_n'
Cuts at: 0 67 112 2880
Size: 67 45 2768
Fragments arranged by size:
2768 67 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2022 2024 2880
Size: 2022 2 856
Fragments arranged by size:
2022 856 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 861 1933 2880
Size: 359 502 1072 947
Fragments arranged by size:
1072 947 502 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1283 2880
Size: 1283 1597
BsaI GGTCTCn'nnnn_
Cuts at: 0 1995 2880
Size: 1995 885
BsaAI yAC'GTr
Cuts at: 0 1063 2204 2880
Size: 1063 1141 676
Fragments arranged by size:
1141 1063 676
BsaWI w'CCGG_w
Cuts at: 0 1034 2880
Size: 1034 1846
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 357 387 944 974 2880
Size: 199 30 128 30 557 30 1906
Fragments arranged by size:
1906 557 199 128 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 835 1371 2880
Size: 835 536 1509
Fragments arranged by size:
1509 835 536
BseYI C'CCAG_C
Cuts at: 0 2828 2832 2880
Size: 2828 4 48
Fragments arranged by size:
2828 48 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2783 2880
Size: 2783 97
BsmBI CGTCTCn'nnnn_
Cuts at: 0 852 2422 2880
Size: 852 1570 458
Fragments arranged by size:
1570 852 458
BspHI T'CATG_A
Cuts at: 0 1277 2111 2880
Size: 1277 834 769
Fragments arranged by size:
1277 834 769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2366 2754 2880
Size: 2366 388 126
Fragments arranged by size:
2366 388 126
BsrDI GCAATG_nn'
Cuts at: 0 2367 2880
Size: 2367 513
BsrGI T'GTAC_A
Cuts at: 0 600 793 2880
Size: 600 193 2087
Fragments arranged by size:
2087 600 193
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1138 1911 2758 2880
Size: 1138 773 847 122
Fragments arranged by size:
1138 847 773 122
BstYI r'GATC_y
Cuts at: 0 573 1008 2041 2728 2824 2880
Size: 573 435 1033 687 96 56
Fragments arranged by size:
1033 687 573 435 96 56
BstZ17I GTA'TAC
Cuts at: 0 84 2880
Size: 84 2796
Bsu36I CC'TnA_GG
Cuts at: 0 170 2880
Size: 170 2710
DraI TTT'AAA
Cuts at: 0 225 1566 2693 2880
Size: 225 1341 1127 187
Fragments arranged by size:
1341 1127 225 187
EaeI y'GGCC_r
Cuts at: 0 2100 2880
Size: 2100 780
EarI CTCTTCn'nnn_
Cuts at: 0 617 698 2354 2880
Size: 617 81 1656 526
Fragments arranged by size:
1656 617 526 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 861 1271 1783 1933 1981 2333 2880
Size: 359 502 410 512 150 48 352 547
Fragments arranged by size:
547 512 502 410 359 352 150 48
EcoO109I rG'GnC_Cy
Cuts at: 0 50 2836 2880
Size: 50 2786 44
Fragments arranged by size:
2786 50 44
EcoRI G'AATT_C
Cuts at: 0 607 2880
Size: 607 2273
EcoRV GAT'ATC
Cuts at: 0 1461 2845 2880
Size: 1461 1384 35
Fragments arranged by size:
1461 1384 35
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 279 1215 2288 2880
Size: 279 936 1073 592
Fragments arranged by size:
1073 936 592 279
HaeII r_GCGC'y
Cuts at: 0 2139 2880
Size: 2139 741
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 357 389 944 976 2880
Size: 357 32 555 32 1904
Fragments arranged by size:
1904 555 357 32 32
HincII GTy'rAC
Cuts at: 0 1178 2880
Size: 1178 1702
HindIII A'AGCT_T
Cuts at: 0 1568 2880
Size: 1568 1312
HpaI GTT'AAC
Cuts at: 0 1178 2880
Size: 1178 1702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1368 2120 2445 2880
Size: 1368 752 325 435
Fragments arranged by size:
1368 752 435 325
MslI CAynn'nnrTG
Cuts at: 0 133 2880
Size: 133 2747
MspA1I CmG'CkG
Cuts at: 0 1911 2832 2880
Size: 1911 921 48
Fragments arranged by size:
1911 921 48
NdeI CA'TA_TG
Cuts at: 0 330 2880
Size: 330 2550
NspI r_CATG'y
Cuts at: 0 780 1109 1620 2484 2512 2577 2880
Size: 780 329 511 864 28 65 303
Fragments arranged by size:
864 780 511 329 303 65 28
PciI A'CATG_T
Cuts at: 0 776 1105 2508 2880
Size: 776 329 1403 372
Fragments arranged by size:
1403 776 372 329
PpuMI rG'GwC_Cy
Cuts at: 0 50 2836 2880
Size: 50 2786 44
Fragments arranged by size:
2786 50 44
PsiI TTA'TAA
Cuts at: 0 2642 2880
Size: 2642 238
PspXI vC'TCGA_Gb
Cuts at: 0 1376 2880
Size: 1376 1504
PstI C_TGCA'G
Cuts at: 0 2445 2880
Size: 2445 435
PvuII CAG'CTG
Cuts at: 0 1911 2832 2880
Size: 1911 921 48
Fragments arranged by size:
1911 921 48
SanDI GG'GwC_CC
Cuts at: 0 50 2880
Size: 50 2830
SfcI C'TryA_G
Cuts at: 0 87 177 1322 2162 2441 2880
Size: 87 90 1145 840 279 439
Fragments arranged by size:
1145 840 439 279 90 87
SmlI C'TyrA_G
Cuts at: 0 1298 1376 2880
Size: 1298 78 1504
Fragments arranged by size:
1504 1298 78
SnaBI TAC'GTA
Cuts at: 0 2204 2880
Size: 2204 676
SspI AAT'ATT
Cuts at: 0 1077 2880
Size: 1077 1803
StyI C'CwwG_G
Cuts at: 0 284 938 1001 1244 2880
Size: 284 654 63 243 1636
Fragments arranged by size:
1636 654 284 243 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 2794 2880
Size: 265 2529 86
Fragments arranged by size:
2529 265 86
TatI w'GTAC_w
Cuts at: 0 600 793 2303 2570 2880
Size: 600 193 1510 267 310
Fragments arranged by size:
1510 600 310 267 193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 2880
Size: 475 32 2373
Fragments arranged by size:
2373 475 32
Tth111I GACn'n_nGTC
Cuts at: 0 958 2880
Size: 958 1922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2836 2880
Size: 2836 44
XhoI C'TCGA_G
Cuts at: 0 1376 2880
Size: 1376 1504
XmnI GAAnn'nnTTC
Cuts at: 0 2730 2880
Size: 2730 150
Enzymes that do cut:
AarI AccI AcuI AflIII AloI AlwNI ApoI AseI
AvaI BamHI BanI BbsI BclI BglI BglII BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsmBI BspHI BspMI BsrDI BsrGI
BstXI BstYI BstZ17I Bsu36I DraI EaeI EarI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI MmeI MslI MspA1I NdeI NspI PciI PpuMI
PsiI PspXI PstI PvuII SanDI SfcI SmlI SnaBI
SspI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BanII
BbeI BbvCI BcgI BciVI BfrBI BlpI Bme1580I BmtI
Bpu10I BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI FseI FspI FspAI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SpeI SphI SrfI StuI SwaI
XbaI XmaI ZraI