Restriction map for
pFN21AE1572
(Linear) (Six-base) MAPSORT of: KIEE1572.seq Check: 2383 from: 1 to: 2580
KIEE1572.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2343 2580
Size: 2343 237
AccI GT'mk_AC
Cuts at: 0 137 493 2580
Size: 137 356 2087
Fragments arranged by size:
2087 356 137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1075 1101 2580
Size: 1075 26 1479
Fragments arranged by size:
1479 1075 26
AfeI AGC'GCT
Cuts at: 0 785 2580
Size: 785 1795
AhdI GACnn_n'nnGTC
Cuts at: 0 2280 2580
Size: 2280 300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2093 2580
Size: 2093 487
AlwNI CAG_nnn'CTG
Cuts at: 0 169 843 1098 1375 2259 2268 2580
Size: 169 674 255 277 884 9 312
Fragments arranged by size:
884 674 312 277 255 169 9
ApaI G_GGCC'C
Cuts at: 0 342 676 2580
Size: 342 334 1904
Fragments arranged by size:
1904 342 334
ApaLI G'TGCA_C
Cuts at: 0 730 2059 2515 2580
Size: 730 1329 456 65
Fragments arranged by size:
1329 730 456 65
ApoI r'AATT_y
Cuts at: 0 796 1030 2239 2580
Size: 796 234 1209 341
Fragments arranged by size:
1209 796 341 234
AvaI C'yCGr_G
Cuts at: 0 319 1969 2580
Size: 319 1650 611
Fragments arranged by size:
1650 611 319
BanI G'GyrC_C
Cuts at: 0 118 2580
Size: 118 2462
BanII G_rGCy'C
Cuts at: 0 342 676 1008 1115 2580
Size: 342 334 332 107 1465
Fragments arranged by size:
1465 342 334 332 107
BbsI GAAGACnn'nnnn_
Cuts at: 0 1414 2580
Size: 1414 1166
BbvCI CC'TCA_GC
Cuts at: 0 255 2580
Size: 255 2325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2227 2261 2580
Size: 2227 34 319
Fragments arranged by size:
2227 319 34
BglI GCCn_nnn'nGGC
Cuts at: 0 174 2580
Size: 174 2406
Bme1580I G_kGCm'C
Cuts at: 0 121 342 676 726 734 2063 2519 2580
Size: 121 221 334 50 8 1329 456 61
Fragments arranged by size:
1329 456 334 221 121 61 50 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1167 2580
Size: 1167 1413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1918 2580
Size: 1918 662
Bpu10I CC'TnA_GC
Cuts at: 0 255 2580
Size: 255 2325
BsaI GGTCTCn'nnnn_
Cuts at: 0 1683 2114 2580
Size: 1683 431 466
Fragments arranged by size:
1683 466 431
BsaAI yAC'GTr
Cuts at: 0 114 2580
Size: 114 2466
BsaBI GATnn'nnATC
Cuts at: 0 7 2580
Size: 7 2573
BsaHI Gr'CG_yC
Cuts at: 0 587 2580
Size: 587 1993
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1661 1691 2580
Size: 1661 30 889
Fragments arranged by size:
1661 889 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 634 1170 1173 1196 2284 2580
Size: 169 465 536 3 23 1088 296
Fragments arranged by size:
1088 536 465 296 169 23 3
BseYI C'CCAG_C
Cuts at: 0 172 684 716 2025 2551 2580
Size: 172 512 32 1309 526 29
Fragments arranged by size:
1309 526 512 172 32 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1913 1999 2308 2580
Size: 493 1420 86 309 272
Fragments arranged by size:
1420 493 309 272 86
BsiEI CG_ry'CG
Cuts at: 0 635 2087 2580
Size: 635 1452 493
Fragments arranged by size:
1452 635 493
BsiHKAI G_wGCw'C
Cuts at: 0 734 1115 2063 2110 2519 2580
Size: 734 381 948 47 409 61
Fragments arranged by size:
948 734 409 381 61 47
BsmBI CGTCTCn'nnnn_
Cuts at: 0 594 1900 2580
Size: 594 1306 680
Fragments arranged by size:
1306 680 594
Bsp1286I G_dGCh'C
Cuts at: 0 121 342 676 726 734 1008 1115 2063
Size: 121 221 334 50 8 274 107 948
Cuts at: 2063 2110 2519 2580
Size: 47 409 61
Fragments arranged by size:
948 409 334 274 221 121 107 61
50 47 8
BspHI T'CATG_A
Cuts at: 0 1212 1283 2580
Size: 1212 71 1297
Fragments arranged by size:
1297 1212 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2177 2225 2343 2580
Size: 2177 48 118 237
Fragments arranged by size:
2177 237 118 48
BsrBI CCG'CTC
Cuts at: 0 42 2580
Size: 42 2538
BsrGI T'GTAC_A
Cuts at: 0 1043 2580
Size: 1043 1537
BssHII G'CGCG_C
Cuts at: 0 246 2580
Size: 246 2334
BssSI C'ACGA_G
Cuts at: 0 2062 2191 2580
Size: 2062 129 389
Fragments arranged by size:
2062 389 129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 843 2067 2268 2580
Size: 843 1224 201 312
Fragments arranged by size:
1224 843 312 201
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 2355 2580
Size: 300 2055 225
Fragments arranged by size:
2055 300 225
BstYI r'GATC_y
Cuts at: 0 954 2580
Size: 954 1626
BtgI C'CryG_G
Cuts at: 0 1997 2201 2210 2580
Size: 1997 204 9 370
Fragments arranged by size:
1997 370 204 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1559 2580
Size: 1559 1021
BtsI GCAGTG_nn'
Cuts at: 0 188 621 2183 2580
Size: 188 433 1562 397
Fragments arranged by size:
1562 433 397 188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2223 2258 2580
Size: 2223 35 322
Fragments arranged by size:
2223 322 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 133 1402 2580
Size: 133 1269 1178
Fragments arranged by size:
1269 1178 133
EaeI y'GGCC_r
Cuts at: 0 528 1125 1262 2084 2159 2198 2369 2580
Size: 528 597 137 822 75 39 171 211
Fragments arranged by size:
822 597 528 211 171 137 75 39
EagI C'GGCC_G
Cuts at: 0 2084 2580
Size: 2084 496
EarI CTCTTCn'nnn_
Cuts at: 0 879 974 1120 1214 1655 1811 1847 2580
Size: 879 95 146 94 441 156 36 733
Fragments arranged by size:
879 733 441 156 146 95 94 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1866 2580
Size: 1866 714
EcoICRI GAG'CTC
Cuts at: 0 1113 2580
Size: 1113 1467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1075 1101 1918 2580
Size: 1075 26 817 662
Fragments arranged by size:
1075 817 662 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 151 1297 1852 2580
Size: 151 1146 555 728
Fragments arranged by size:
1146 728 555 151
EcoO109I rG'GnC_Cy
Cuts at: 0 105 338 393 597 673 1644 1871 2580
Size: 105 233 55 204 76 971 227 709
Fragments arranged by size:
971 709 233 227 204 105 76 55
EcoRI G'AATT_C
Cuts at: 0 796 2580
Size: 796 1784
EcoRV GAT'ATC
Cuts at: 0 713 2580
Size: 713 1867
HaeII r_GCGC'y
Cuts at: 0 787 2009 2047 2580
Size: 787 1222 38 533
Fragments arranged by size:
1222 787 533 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 902 934 2266 2298 2580
Size: 902 32 1332 32 282
Fragments arranged by size:
1332 902 282 32 32
HincII GTy'rAC
Cuts at: 0 75 306 494 2580
Size: 75 231 188 2086
Fragments arranged by size:
2086 231 188 75
HindIII A'AGCT_T
Cuts at: 0 2446 2580
Size: 2446 134
MfeI C'AATT_G
Cuts at: 0 1740 2580
Size: 1740 840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 719 800 1647 2454 2580
Size: 131 588 81 847 807 126
Fragments arranged by size:
847 807 588 131 126 81
MscI TGG'CCA
Cuts at: 0 1264 2161 2371 2580
Size: 1264 897 210 209
Fragments arranged by size:
1264 897 210 209
MslI CAynn'nnrTG
Cuts at: 0 445 2353 2580
Size: 445 1908 227
Fragments arranged by size:
1908 445 227
MspA1I CmG'CkG
Cuts at: 0 204 1236 2083 2580
Size: 204 1032 847 497
Fragments arranged by size:
1032 847 497 204
NcoI C'CATG_G
Cuts at: 0 1997 2580
Size: 1997 583
NspI r_CATG'y
Cuts at: 0 312 693 819 2176 2580
Size: 312 381 126 1357 404
Fragments arranged by size:
1357 404 381 312 126
PasI CC'CwG_GG
Cuts at: 0 1242 2580
Size: 1242 1338
PflMI CCAn_nnn'nTGG
Cuts at: 0 2196 2506 2580
Size: 2196 310 74
Fragments arranged by size:
2196 310 74
PfoI T'CCnGG_A
Cuts at: 0 1097 1139 1550 2580
Size: 1097 42 411 1030
Fragments arranged by size:
1097 1030 411 42
PpuMI rG'GwC_Cy
Cuts at: 0 105 393 597 1644 2580
Size: 105 288 204 1047 936
Fragments arranged by size:
1047 936 288 204 105
PsiI TTA'TAA
Cuts at: 0 1493 2580
Size: 1493 1087
PspOMI G'GGCC_C
Cuts at: 0 338 672 2580
Size: 338 334 1908
Fragments arranged by size:
1908 338 334
PspXI vC'TCGA_Gb
Cuts at: 0 319 2580
Size: 319 2261
PstI C_TGCA'G
Cuts at: 0 209 616 1061 1515 1652 1811 1868 1982
Size: 209 407 445 454 137 159 57 114
Cuts at: 1982 2072 2264 2580
Size: 90 192 316
Fragments arranged by size:
454 445 407 316 209 192 159 137
114 90 57
PvuI CG_AT'CG
Cuts at: 0 635 2580
Size: 635 1945
PvuII CAG'CTG
Cuts at: 0 204 1236 2580
Size: 204 1032 1344
Fragments arranged by size:
1344 1032 204
SacI G_AGCT'C
Cuts at: 0 1115 2580
Size: 1115 1465
SalI G'TCGA_C
Cuts at: 0 492 2580
Size: 492 2088
SanDI GG'GwC_CC
Cuts at: 0 393 2580
Size: 393 2187
SapI GCTCTTCn'nnn_
Cuts at: 0 1120 2580
Size: 1120 1460
SexAI A'CCwGG_T
Cuts at: 0 2278 2580
Size: 2278 302
SfcI C'TryA_G
Cuts at: 0 205 612 868 1057 1511 1648 1807 1864
Size: 205 407 256 189 454 137 159 57
Cuts at: 1864 1978 2068 2260 2580
Size: 114 90 192 320
Fragments arranged by size:
454 407 320 256 205 192 189 159
137 114 90 57
SmlI C'TyrA_G
Cuts at: 0 319 1969 2580
Size: 319 1650 611
Fragments arranged by size:
1650 611 319
SpeI A'CTAG_T
Cuts at: 0 1620 2580
Size: 1620 960
SphI G_CATG'C
Cuts at: 0 2176 2580
Size: 2176 404
StyI C'CwwG_G
Cuts at: 0 1997 2580
Size: 1997 583
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1515 1843 2536 2580
Size: 1515 328 693 44
Fragments arranged by size:
1515 693 328 44
TatI w'GTAC_w
Cuts at: 0 560 1043 2580
Size: 560 483 1537
Fragments arranged by size:
1537 560 483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1609 1641 2580
Size: 1609 32 939
Fragments arranged by size:
1609 939 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1692 2580
Size: 1692 888
XhoI C'TCGA_G
Cuts at: 0 319 1969 2580
Size: 319 1650 611
Fragments arranged by size:
1650 611 319
XmnI GAAnn'nnTTC
Cuts at: 0 1031 2580
Size: 1031 1549
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BglI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI CspCI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SapI SexAI SfcI SmlI SpeI SphI StyI
TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI
BsaWI BsiWI BsmI BspEI BsrDI BsrFI BstBI BstEII
BstZ17I Bsu36I ClaI DraI DraIII FalI FseI FspI
FspAI HpaI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsrI RsrII SacII SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI
StuI SwaI Tth111I XbaI XmaI ZraI